Q pili enhance the attachment of Moraxella bovis to bovine corneas in vitro

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dc.contributor.author Ruehl, W. W. en_US
dc.contributor.author Marrs, C. en_US
dc.contributor.author Beard, M. K. en_US
dc.contributor.author Shokooki, V. en_US
dc.contributor.author Hinojoza, J. R. en_US
dc.contributor.author Banks, S. en_US
dc.contributor.author Bieber, D. en_US
dc.contributor.author Mattick, J. S. en_US
dc.date.accessioned 2010-06-01T19:36:33Z
dc.date.available 2010-06-01T19:36:33Z
dc.date.issued 1993-01 en_US
dc.identifier.citation Ruehl, W. W.; Marrs, C.; Beard, M. K.; Shokooki, V.; Hinojoza, J. R.; Banks, S.; Bieber, D.; Mattick, J. S. (1993). "Q pili enhance the attachment of Moraxella bovis to bovine corneas in vitro ." Molecular Microbiology 7(2): 285-288. <http://hdl.handle.net/2027.42/72745> en_US
dc.identifier.issn 0950-382X en_US
dc.identifier.issn 1365-2958 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/72745
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8095318&dopt=citation en_US
dc.description.abstract Moraxella bovis , the causative agent of infectious bovine keratoconjunctivttis, exhibits several virulence factors, including pili, haemotysin, leukotoxin, and proteases. The pili are filamentous appendages which mediate bacterial adherence. Prior studies have shown that Q-piliated M. bovis Epp63 are more infectious and more pathogenic than l-piliated and nonpiliated isogenic variants, suggesting that Q pili perse or traits associated with Q-pilin expression, promote the early association of Q-pillated bacteria with bovine corneal tissue. In order to better evaluate the role of Q pili in M. bovis attachment, several M. bovis strains and a recombinant P. aeruginosa strain which elaborates M. bovis Q pili but not P. aeruginosa PAK pili, were evaluated using an in vitro corneal attachment assay. For each strain tested, piliated organisms attached better than non-piliated bacteria. M. bovis Epp63 Q-piIiated bacteria adhered better than either the l-piliated or non-piliated isogenic variants. Finally, recombinant P. aeruginosa organisms elaborating M. bovis Q pili adhered better than the parent P. aeruginosa strain which did not produce M. bovis pili. These results indicate that the presence of pili, especially Q pili, enhances the attachment of bacteria to bovine cornea In vitro. en_US
dc.format.extent 1972161 bytes
dc.format.extent 3109 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.publisher Blackwell Publishing Ltd en_US
dc.rights 1993 Blackwell Publishing en_US
dc.title Q pili enhance the attachment of Moraxella bovis to bovine corneas in vitro en_US
dc.type Article en_US
dc.subject.hlbsecondlevel Microbiology and Immunology en_US
dc.subject.hlbtoplevel Science en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Epidemiology, School of Epidemiology and Public Health, University of Michigan, Ann Arbor, Michigan, USA. en_US
dc.contributor.affiliationother Department of Pathology, Stanford University School of Medicine, Stanford, California USA. en_US
dc.contributor.affiliationother Department of Comparative Medicine, Stanford University School of Medicine, Stanford, California USA. en_US
dc.contributor.affiliationother Department of Animal Health, The University of Sydney, Camden, New South Wales, Australia. en_US
dc.contributor.affiliationother Centre for Molecular Biology and Biotechnology, University of Queensland, St Lucia, Queensland, Australia. en_US
dc.identifier.pmid 8095318 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/72745/1/j.1365-2958.1993.tb01119.x.pdf
dc.identifier.doi 10.1111/j.1365-2958.1993.tb01119.x en_US
dc.identifier.source Molecular Microbiology en_US
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dc.owningcollname Interdisciplinary and Peer-Reviewed
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