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Targeted disruption of the PD78 gene ( traF ) reduces pheromone-inducible conjugal transfer of the bacteriocin plasmid pPD1 in Enterococcus faecalis
dc.contributor.authorNakayama, Jiroen_US
dc.contributor.authorClewell, Don B.en_US
dc.contributor.authorSuzuki, Akinorien_US
dc.date.accessioned2010-06-01T19:43:00Z
dc.date.available2010-06-01T19:43:00Z
dc.date.issued1995-05en_US
dc.identifier.citationNakayama, Jiro; Clewell, Don B.; Suzuki, Akinori (1995). "Targeted disruption of the PD78 gene ( traF ) reduces pheromone-inducible conjugal transfer of the bacteriocin plasmid pPD1 in Enterococcus faecalis ." FEMS Microbiology Letters 128(3): 283-288. <http://hdl.handle.net/2027.42/72850>en_US
dc.identifier.issn0378-1097en_US
dc.identifier.issn1574-6968en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/72850
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7781976&dopt=citationen_US
dc.description.abstractBacterial sex pheromone, cPD1, induces sexual aggregation of Enterococcus faecalis harboring the bacteriocin plasmid, pPD1, and enables pPD1 to transfer at high frequency in a liquid culture. PD78 is a cPD1-inducible cell surface protein encoded by pPD1. The PD78 gene, traF , was disrupted by homologous recombination between pPD1 and an artificial vector having a deletion in the middle portion of traF . The disruption of traF did not affect the cPD1-inducible aggregation but reduced the transfer frequency of pPD1 to 2% of the wild-type level.en_US
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dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rights1995 Federation of European Microbiological Societiesen_US
dc.subject.otherEnterococcus Faecalisen_US
dc.subject.otherConjugative Plasmiden_US
dc.subject.otherGene Disruptionen_US
dc.subject.otherBacterial Sex Pheromoneen_US
dc.subject.otherTraFen_US
dc.subject.otherPPDlen_US
dc.titleTargeted disruption of the PD78 gene ( traF ) reduces pheromone-inducible conjugal transfer of the bacteriocin plasmid pPD1 in Enterococcus faecalisen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biologic and Materials Sciences, School of Dentistry and Department of Microbiology /Immunology, School of Medicine, The University of Michigan, Ann Arbor, MI 48109-1078, USAen_US
dc.identifier.pmid7781976en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/72850/1/j.1574-6968.1995.tb07537.x.pdf
dc.identifier.doi10.1111/j.1574-6968.1995.tb07537.xen_US
dc.identifier.sourceFEMS Microbiology Lettersen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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