Cellular localization of a Hsp90 homologue in Porphyromonas gingivalis
dc.contributor.author | Lopatin, Dennis E. | en_US |
dc.contributor.author | Jaramillo, Eduardo | en_US |
dc.contributor.author | Edwards, Chris A. | en_US |
dc.contributor.author | Poperin, Neal | en_US |
dc.contributor.author | Combs, Allison | en_US |
dc.contributor.author | Shelburne, Charles E. | en_US |
dc.date.accessioned | 2010-06-01T19:43:22Z | |
dc.date.available | 2010-06-01T19:43:22Z | |
dc.date.issued | 1999-12 | en_US |
dc.identifier.citation | Lopatin, Dennis E.; Jaramillo, Eduardo; Edwards, Chris A.; Poperin, Neal; Combs, Allison; Shelburne, Charles E. (1999). "Cellular localization of a Hsp90 homologue in Porphyromonas gingivalis ." FEMS Microbiology Letters 181(1): 9-16. <http://hdl.handle.net/2027.42/72856> | en_US |
dc.identifier.issn | 0378-1097 | en_US |
dc.identifier.issn | 1574-6968 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/72856 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10564783&dopt=citation | en_US |
dc.description.abstract | We previously reported an association between elevated serum antibody titers to the 90-kDa human heat shock protein (Hsp90), periodontal health and colonization by Porphyromonas gingivalis . In this study, we examined the cellular localization of the Hsp90 homologue of P. gingivalis . Cultures of P. gingivalis were heat-stressed (45°C) and examined for localization of the Hsp90 homologue. Heat stress induced a 4–5-fold increase in anti-Hsp90 antibody reactivity over that of the unstressed controls. Western blot analysis revealed two bands (44 and 68 kDa) that reacted with anti-Hsp90 antibodies. The 68-kDa band was heat-inducible, while the 44-kDa band was not. Immunogold staining revealed that the Hsp90 homologue localized principally to the membrane and extracellular vesicles. Subcellular fractionation confirmed that the Hsp90 homologue was primarily membrane-associated. | en_US |
dc.format.extent | 367737 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1999 Federation of European Microbiological Societies | en_US |
dc.subject.other | Heat Shock | en_US |
dc.subject.other | Extracellular Vesicle | en_US |
dc.subject.other | 90-kDa Human Heat Shock Protein | en_US |
dc.subject.other | Porphyromonas Gingivalis | en_US |
dc.title | Cellular localization of a Hsp90 homologue in Porphyromonas gingivalis | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biologic and Materials Science, University of Michigan School of Dentistry, 1011 North University Ave, Campus Box 1078, Ann Arbor, MI 48109-1078, USA | en_US |
dc.contributor.affiliationother | BioMaterials Technology Center, 3M, St. Paul, MN, USA | en_US |
dc.identifier.pmid | 10564783 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/72856/1/j.1574-6968.1999.tb08820.x.pdf | |
dc.identifier.doi | 10.1111/j.1574-6968.1999.tb08820.x | en_US |
dc.identifier.source | FEMS Microbiology Letters | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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