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The role of laccase in prostaglandin production by Cryptococcus neoformans
dc.contributor.authorErb-Downward, John R.en_US
dc.contributor.authorNoggle, Rachael M.en_US
dc.contributor.authorWilliamson, Peter R.en_US
dc.contributor.authorHuffnagle, Gary B.en_US
dc.date.accessioned2010-06-01T19:54:42Z
dc.date.available2010-06-01T19:54:42Z
dc.date.issued2008-06en_US
dc.identifier.citationErb-Downward, John R.; Noggle, Rachael M.; Williamson, Peter R.; Huffnagle, Gary B. (2008). "The role of laccase in prostaglandin production by Cryptococcus neoformans ." Molecular Microbiology 68(6): 1428-1437. <http://hdl.handle.net/2027.42/73041>en_US
dc.identifier.issn0950-382Xen_US
dc.identifier.issn1365-2958en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/73041
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18410494&dopt=citationen_US
dc.description.abstractRecently, it has been demonstrated that the opportunistic fungal pathogen Cryptococcus neoformans can synthesize authentic immunomodulatory prostaglandins. The mechanism by which this takes place is unclear as there is no cyclooxygenase homologue in the cryptococcal genome. In this study, we show that cryptococcal production of both PGE 2 and PGF 2α can be chemically inhibited by caffeic acid, resveratrol and nordihydroguaiaretic acid. These polyphenolic molecules are frequently used as inhibitors of lipoxygenase enzymes; however, blast searches of the cryptococcal genome were unable to identify any homologues of mammalian, plant or fungal lipoxygenases. Next we investigated cryptococcal laccase, an enzyme known to bind polyphenols, and found that either antibody depletion or genetic deletion of the primary cryptococcal laccase ( lac1 δ) resulted in a loss of cryptococcal prostaglandin production. To determine how laccase is involved, we tested recombinant laccase activity on the prostaglandin precursors, arachidonic acid (AA), PGG 2 and PGH 2 . Using mass spectroscopy we determined that recombinant Lac1 does not modify AA or PGH 2 , but does have a marked activity toward PGG 2 converting it to PGE 2 and 15-keto-PGE 2 . These data demonstrate a critical role for laccase in cryptococcal prostaglandin production, and provides insight into a new and unique fungal prostaglandin pathway.en_US
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dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rightsJournal compilation © 2008 Blackwell Publishingen_US
dc.titleThe role of laccase in prostaglandin production by Cryptococcus neoformansen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109-0642, USA.en_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-0642, USA.en_US
dc.contributor.affiliationotherSection of Infectious Diseases, Department of Medicine, University of Illinois at Chicago, Chicago, IL, USA.en_US
dc.identifier.pmid18410494en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/73041/1/j.1365-2958.2008.06245.x.pdf
dc.identifier.doi10.1111/j.1365-2958.2008.06245.xen_US
dc.identifier.sourceMolecular Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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