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Use of the gyrB gene for the identification of Pandoraea species

dc.contributor.authorCoenye, Tomen_US
dc.contributor.authorLiPuma, John J.en_US
dc.date.accessioned2010-06-01T20:02:40Z
dc.date.available2010-06-01T20:02:40Z
dc.date.issued2002-02en_US
dc.identifier.citationCoenye, Tom; LiPuma, John J (2002). "Use of the gyrB gene for the identification of Pandoraea species." FEMS Microbiology Letters 208(1): 15-19. <http://hdl.handle.net/2027.42/73171>en_US
dc.identifier.issn0378-1097en_US
dc.identifier.issn1574-6968en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/73171
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=11934487&dopt=citationen_US
dc.description.abstractThe recently described genus Pandoraea consists of five named species and four unnamed genomospecies, several of which have been identified in clinical specimens including respiratory secretions from persons with cystic fibrosis. We investigated whether it is possible to distinguish species of the genus Pandoraea by means of restriction fragment length polymorphism (RFLP) analysis and direct sequencing of the gyrB gene. Sixty-seven Pandoraea isolates were included. Species-specific RFLP patterns were obtained following digestion of the PCR-amplified gyrB gene with Msp I. Specificity of RFLP groupings was confirmed by direct sequencing of several representative isolates. Our results indicate that RFLP analysis and sequencing of the gyrB gene are useful for the identification of Pandoraea species. We also found that further taxonomic studies within the Β- Proteobacteria using the gyrB gene would benefit from the development of additional primers allowing more efficient amplification of the gyrB gene. Our data also indicate that the taxonomic status of Pandoraea genomospecies 2 should be reinvestigated.en_US
dc.format.extent331831 bytes
dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rights2002 Federation of European Microbiological Societiesen_US
dc.subject.otherGyrBen_US
dc.subject.otherIdentificationen_US
dc.subject.otherCystic Fibrosisen_US
dc.subject.otherPandoraeaen_US
dc.subject.otherBurkholderia Cepacia Complexen_US
dc.titleUse of the gyrB gene for the identification of Pandoraea speciesen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pediatrics and Communicable Diseases, University of Michigan Medical School, 8301 MSRB III, Box 0646, 1150 W. Med. Ctr. Dr., Ann Arbor, MI 48109-0646, USAen_US
dc.identifier.pmid11934487en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/73171/1/j.1574-6968.2002.tb11053.x.pdf
dc.identifier.doi10.1111/j.1574-6968.2002.tb11053.xen_US
dc.identifier.sourceFEMS Microbiology Lettersen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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