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Localization of Phosphatidylinositol 4,5-P 2 Important in Exocytosis and a Quantitative Analysis of Chromaffin Granule Motion Adjacent to the Plasma Membrane
dc.contributor.authorHolz, Ronald W.en_US
dc.contributor.authorAxelrod, Danielen_US
dc.date.accessioned2010-06-01T20:17:40Z
dc.date.available2010-06-01T20:17:40Z
dc.date.issued2002-10en_US
dc.identifier.citationHOLZ, RONALD W.; AXELROD, DANIEL (2002). "Localization of Phosphatidylinositol 4,5-P 2 Important in Exocytosis and a Quantitative Analysis of Chromaffin Granule Motion Adjacent to the Plasma Membrane." Annals of the New York Academy of Sciences 971(1 THE CHROMAFFIN CELL: TRANSMITTER BIOSYNTHESIS, STORAGE, RELEASE, ACTIONS, AND INFORMATICS: 11th INTERNATIONAL SYMPOSIUM ON CHROMAFFIN CELL BIOLOGY ): 232-243. <http://hdl.handle.net/2027.42/73413>en_US
dc.identifier.issn0077-8923en_US
dc.identifier.issn1749-6632en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/73413
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=12438123&dopt=citationen_US
dc.description.abstractA slow ATP-dependent priming step precedes a rapid, Ca 2+ -dependent triggering step in exocytosis in chromaffin cells and in most, if not all, differentiated secretory cells. A major component of ATP-dependent secretion in permeabilized cells reflects the maintenance of the polyphosphoinositides, especially PtdIns-4,5-P2. Here we summarize recent experiments with PH-GFP (binds to PtdIns-4,5-P2) that indicate that PtdIns-4,5-P2 is localized primarily on the plasma membrane in chromaffin cells, and that it is this pool that plays a role in exocytosis. It is demonstrated that transiently expressed PH-GFP inhibits secretion in subsequently permeabilized cells. Recent studies using total internal reflection fluorescent microscopy (TIRFM) to measure chromaffin granule motion adjacent to the plasma membrane are also summarized. The quantitative analysis indicates that chromaffin granule motion is highly restricted and suggests that chromaffin granules are caged or tethered immediately adjacent to the plasma membrane.en_US
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dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rights2002 New York Academy of Sciencesen_US
dc.subject.otherPhosphatidylinositol 4,5-P 2en_US
dc.subject.otherExocytosisen_US
dc.subject.otherChromaffin Granule Motionen_US
dc.subject.otherPleckstrin Homology Domainen_US
dc.subject.otherGreen Fluorescent Proteinen_US
dc.subject.otherPro-atrial Natiuretic Peptideen_US
dc.titleLocalization of Phosphatidylinositol 4,5-P 2 Important in Exocytosis and a Quantitative Analysis of Chromaffin Granule Motion Adjacent to the Plasma Membraneen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelScience (General)en_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pharmacology, Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationumDepartment of Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.identifier.pmid12438123en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/73413/1/j.1749-6632.2002.tb04467.x.pdf
dc.identifier.doi10.1111/j.1749-6632.2002.tb04467.xen_US
dc.identifier.sourceAnnals of the New York Academy of Sciencesen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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