Cloning, expression and functional characterization of the full-length murine ADAMTS13
dc.contributor.author | Bruno, K. | en_US |
dc.contributor.author | Völkel, D. | en_US |
dc.contributor.author | Plaimauer, B. | en_US |
dc.contributor.author | Antoine, G. | en_US |
dc.contributor.author | Pable, S. | en_US |
dc.contributor.author | Motto, D. G. | en_US |
dc.contributor.author | Lemmerhirt, H. L. | en_US |
dc.contributor.author | Dorner, F. | en_US |
dc.contributor.author | Zimmermann, K. | en_US |
dc.contributor.author | Scheiflinger, F. | en_US |
dc.date.accessioned | 2010-06-01T20:28:48Z | |
dc.date.available | 2010-06-01T20:28:48Z | |
dc.date.issued | 2005-05 | en_US |
dc.identifier.citation | BRUNO, K.; VÖLKEL, D.; PLAIMAUER, B.; ANTOINE, G.; PABLE, S.; MOTTO, D. G.; LEMMERHIRT, H. L.; DORNER, F.; ZIMMERMANN, K.; SCHEIFLINGER, F. (2005). "Cloning, expression and functional characterization of the full-length murine ADAMTS13 ." Journal of Thrombosis and Haemostasis 3(5): 1064-1073. <http://hdl.handle.net/2027.42/73592> | en_US |
dc.identifier.issn | 1538-7933 | en_US |
dc.identifier.issn | 1538-7836 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/73592 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=15869605&dopt=citation | en_US |
dc.description.abstract | Functional deficiency or absence of the human von Willebrand factor (VWF)-cleaving protease (VWF-cp), recently termed ADAMTS13, has been shown to cause acquired and congenital thrombotic thrombocytopenic purpura (TTP), respectively. As a first step towards developing a small animal model of TTP, we have cloned the complete (non-truncated) murine Adamts13 gene from BALB/c mice liver poly A + mRNA. Murine ADAMTS13 is a 1426-amino-acid protein with a high homology and similar structural organization to the human ortholog. Transient expression of the murine Adamts13 cDNA in HEK 293 cells yielded a protein with a molecular weight of approximately 180 kDa which degraded recombinant murine VWF (rVWF) in a dose-dependent manner. The cleavage products of murine rVWF had the expected size of 140 and 170 kDa. Murine ADAMTS13 was inhibited by EDTA and the plasma from a TTP patient. | en_US |
dc.format.extent | 797917 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Science Inc | en_US |
dc.rights | 2005 International Society on Thrombosis and Haemostasis | en_US |
dc.subject.other | ADAMTS13 | en_US |
dc.subject.other | Thrombotic Thrombocytopenic Purpura | en_US |
dc.subject.other | Von Willebrand Factor | en_US |
dc.subject.other | Von Willebrand Factor-cleaving Protease | en_US |
dc.title | Cloning, expression and functional characterization of the full-length murine ADAMTS13 | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | † University of Michigan, Department of Pediatrics, Life Sciences Institute, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | * Baxter BioScience, Biomedical Research Center, Orth/Donau, Austria | en_US |
dc.identifier.pmid | 15869605 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/73592/1/j.1538-7836.2005.01246.x.pdf | |
dc.identifier.doi | 10.1111/j.1538-7836.2005.01246.x | en_US |
dc.identifier.source | Journal of Thrombosis and Haemostasis | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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