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Cloning, expression and functional characterization of the full-length murine ADAMTS13

dc.contributor.authorBruno, K.en_US
dc.contributor.authorVölkel, D.en_US
dc.contributor.authorPlaimauer, B.en_US
dc.contributor.authorAntoine, G.en_US
dc.contributor.authorPable, S.en_US
dc.contributor.authorMotto, D. G.en_US
dc.contributor.authorLemmerhirt, H. L.en_US
dc.contributor.authorDorner, F.en_US
dc.contributor.authorZimmermann, K.en_US
dc.contributor.authorScheiflinger, F.en_US
dc.date.accessioned2010-06-01T20:28:48Z
dc.date.available2010-06-01T20:28:48Z
dc.date.issued2005-05en_US
dc.identifier.citationBRUNO, K.; VÖLKEL, D.; PLAIMAUER, B.; ANTOINE, G.; PABLE, S.; MOTTO, D. G.; LEMMERHIRT, H. L.; DORNER, F.; ZIMMERMANN, K.; SCHEIFLINGER, F. (2005). "Cloning, expression and functional characterization of the full-length murine ADAMTS13 ." Journal of Thrombosis and Haemostasis 3(5): 1064-1073. <http://hdl.handle.net/2027.42/73592>en_US
dc.identifier.issn1538-7933en_US
dc.identifier.issn1538-7836en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/73592
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=15869605&dopt=citationen_US
dc.description.abstractFunctional deficiency or absence of the human von Willebrand factor (VWF)-cleaving protease (VWF-cp), recently termed ADAMTS13, has been shown to cause acquired and congenital thrombotic thrombocytopenic purpura (TTP), respectively. As a first step towards developing a small animal model of TTP, we have cloned the complete (non-truncated) murine Adamts13 gene from BALB/c mice liver poly A + mRNA. Murine ADAMTS13 is a 1426-amino-acid protein with a high homology and similar structural organization to the human ortholog. Transient expression of the murine Adamts13 cDNA in HEK 293 cells yielded a protein with a molecular weight of approximately 180 kDa which degraded recombinant murine VWF (rVWF) in a dose-dependent manner. The cleavage products of murine rVWF had the expected size of 140 and 170 kDa. Murine ADAMTS13 was inhibited by EDTA and the plasma from a TTP patient.en_US
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dc.publisherBlackwell Science Incen_US
dc.rights2005 International Society on Thrombosis and Haemostasisen_US
dc.subject.otherADAMTS13en_US
dc.subject.otherThrombotic Thrombocytopenic Purpuraen_US
dc.subject.otherVon Willebrand Factoren_US
dc.subject.otherVon Willebrand Factor-cleaving Proteaseen_US
dc.titleCloning, expression and functional characterization of the full-length murine ADAMTS13en_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelInternal Medicine and Specialtiesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationum† University of Michigan, Department of Pediatrics, Life Sciences Institute, Ann Arbor, MI, USAen_US
dc.contributor.affiliationother* Baxter BioScience, Biomedical Research Center, Orth/Donau, Austriaen_US
dc.identifier.pmid15869605en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/73592/1/j.1538-7836.2005.01246.x.pdf
dc.identifier.doi10.1111/j.1538-7836.2005.01246.xen_US
dc.identifier.sourceJournal of Thrombosis and Haemostasisen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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