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Membrane localization of the ToxR winged-helix domain is required for TcpP-mediated virulence gene activation in Vibrio cholerae
dc.contributor.authorCrawford, J. Adamen_US
dc.contributor.authorKrukonis, Eric S.en_US
dc.contributor.authorDiRita, Victor J.en_US
dc.date.accessioned2010-06-01T20:44:53Z
dc.date.available2010-06-01T20:44:53Z
dc.date.issued2003-03en_US
dc.identifier.citationCrawford, J. Adam; Krukonis, Eric S.; DiRita, Victor J. (2003). "Membrane localization of the ToxR winged-helix domain is required for TcpP-mediated virulence gene activation in Vibrio cholerae ." Molecular Microbiology 47(5): 1459-1473. <http://hdl.handle.net/2027.42/73850>en_US
dc.identifier.issn0950-382Xen_US
dc.identifier.issn1365-2958en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/73850
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=12603748&dopt=citationen_US
dc.description.abstractToxR is a bitopic membrane protein that controls virulence gene expression in Vibrio cholerae . Its cytoplasmic domain is homologous to the winged helix–turn–helix (‘winged helix’) DNA-binding/transcription activation domain found in a variety of prokaryotic and eukaryotic regulators, whereas its periplasmic domain is of ill-defined function. Several genes in V. cholerae are regulated by ToxR, but by apparently different mechanisms. Whereas ToxR directly controls the transcription of genes encoding two outer membrane proteins, OmpU and OmpT, it co-operates with a second membrane-localized transcription factor called TcpP to activate transcription of the gene encoding ToxT, which regulates transcription of cholera toxin ( ctxAB ) and the toxin-co-regulated pilus ( tcp ). To determine the requirements for gene activation by ToxR, different domains of the protein were analysed for their ability to control expression of toxT , ompU and ompT . Soluble forms of the cytoplasmic winged-helix domain regulated ompU and ompT gene expression properly but did not activate toxT transcription. Membrane localization of the winged helix was sufficient for both omp gene regulation and TcpP-dependent toxT transcription, irrespective of the type of periplasmic domain or even the presence of a periplasmic domain. These results suggest that (i) the major function for membrane localization of ToxR is for its winged-helix domain to co-operate with TcpP to activate transcription; (ii) the periplasmic domain of ToxR is not required for TcpP-dependent activation of toxT transcription; and (iii) membrane localization is not a strict requirement for DNA binding and transcription activation by ToxR.en_US
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dc.publisherBlackwell Science Ltden_US
dc.rightsBlackwell Publishing Ltd, 2003en_US
dc.titleMembrane localization of the ToxR winged-helix domain is required for TcpP-mediated virulence gene activation in Vibrio choleraeen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumUnit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, MI 48109, USA.en_US
dc.contributor.affiliationotherDepartment of Microbiology and Immunology, anden_US
dc.identifier.pmid12603748en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/73850/1/j.1365-2958.2003.03398.x.pdf
dc.identifier.doi10.1046/j.1365-2958.2003.03398.xen_US
dc.identifier.sourceMolecular Microbiologyen_US
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