Legionella pneumophila CsrA is a pivotal repressor of transmission traits and activator of replication
dc.contributor.author | Molofsky, Ari B. | en_US |
dc.contributor.author | Swanson, Michele S. | en_US |
dc.date.accessioned | 2010-06-01T20:57:40Z | |
dc.date.available | 2010-06-01T20:57:40Z | |
dc.date.issued | 2003-10 | en_US |
dc.identifier.citation | Molofsky, Ari B.; Swanson, Michele S. (2003). " Legionella pneumophila CsrA is a pivotal repressor of transmission traits and activator of replication." Molecular Microbiology 50(2): 445-461. <http://hdl.handle.net/2027.42/74055> | en_US |
dc.identifier.issn | 0950-382X | en_US |
dc.identifier.issn | 1365-2958 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/74055 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=14617170&dopt=citation | en_US |
dc.description.abstract | Legionella pneumophila can replicate inside amoebae and also alveolar macrophages to cause Legionnaires’ Disease in susceptible hosts. When nutrients become limiting, a stringent-like response coordinates the differentiation of L. pneumophila to a transmissive form, a process mediated by the two-component system LetA/S and the sigma factors RpoS and FliA. Here we demonstrate that the broadly conserved RNA binding protein CsrA is a global repressor of L. pneumophila transmission phenotypes and an essential activator of intracellular replication. By analysing csrA expression and the phenotypes of csrA single and double mutants and a strain that expresses csrA constitutively, we demonstrate that, during replication in broth, CsrA represses every post-exponential phase phenotype examined, including cell shape shortening, motility, pigmentation, stress resistance, sodium sensitivity, cytotoxicity and efficient macrophage infection. At the transition to the post-exponential phase, LetA/S relieves CsrA repression to induce transmission phenotypes by both FliA-dependent and -independent pathways. For L. pneumophila to avoid lysosomal degradation in macrophages, CsrA repression must be relieved by LetA/S before phagocytosis; conversely, before intracellular bacteria can replicate, CsrA repression must be restored. The reciprocal regulation of replication and transmission exemplified by CsrA likely enhances the fitness of microbes faced with fluctuating environments. | en_US |
dc.format.extent | 418251 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Science Ltd | en_US |
dc.rights | 2003 Blackwell Publishing Ltd | en_US |
dc.title | Legionella pneumophila CsrA is a pivotal repressor of transmission traits and activator of replication | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109–0620, USA. | en_US |
dc.identifier.pmid | 14617170 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/74055/1/j.1365-2958.2003.03706.x.pdf | |
dc.identifier.doi | 10.1046/j.1365-2958.2003.03706.x | en_US |
dc.identifier.source | Molecular Microbiology | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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