Method for the Simultaneous Establishment of Many Axenic Cultures of Paramecium * †
dc.contributor.author | Allen, Sally Lyman | en_US |
dc.contributor.author | Nerad, Thomas A. | en_US |
dc.date.accessioned | 2010-06-01T21:11:57Z | |
dc.date.available | 2010-06-01T21:11:57Z | |
dc.date.issued | 1978-02 | en_US |
dc.identifier.citation | ALLEN, SALLY LYMAN; NERAD, THOMAS A. (1978). "Method for the Simultaneous Establishment of Many Axenic Cultures of Paramecium * † ." Journal of Eukaryotic Microbiology 25(1): 134-139. <http://hdl.handle.net/2027.42/74279> | en_US |
dc.identifier.issn | 1066-5234 | en_US |
dc.identifier.issn | 1550-7408 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/74279 | |
dc.description.abstract | A method is described for the simultaneous treatment of 42 (or more) stocks of Paramecium , and their adaptation to growth in axenic culture. Samples of dense cultures of these ciliates growing with Enterobacter aerogenes are rendered bacteria-free by migration through 2 sets of tubes containing Adaptation Medium (Peters' salts solution, stigmasterol, vitamins, and autoclaved E. aerogenes ). The 2nd set of tubes contains Adaptation Medium plus antibiotics. Bacteria-free samples containing ∼ 100 animals are then transferred to test tubes containing Adaptation Medium without antibiotics. This medium also serves as a growth medium. It supports indefinite growth of all Paramecium stocks tested. After adaptation to this medium, the ciliates can be grown in the axenic medium developed by Soldo, Godoy & van Wagtendonk. On a single trial at least half of the stocks can be expected to produce axenic cultures within 5 to 10 days by these procedures. The method has been applied successfully to several of the species of the Paramecium aurelia complex, to all syngens of Paramecium multimicronucleatum , to several stocks of Paramecium jenningsi , and to 1 stock each of Paramecium caudatum and Paramecium calkinsi. A modification of the method also works for Didinium nasutum. | en_US |
dc.format.extent | 604804 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1978 by the Society of Protozoologists | en_US |
dc.subject.other | Paramecium Aurelia Complex | en_US |
dc.subject.other | Paramecium Multimicronucleatum | en_US |
dc.subject.other | Paramecium Jenningsi | en_US |
dc.subject.other | Paramecium Caudatum | en_US |
dc.subject.other | Paramecium Calkinsi | en_US |
dc.subject.other | Didinium Nasutum | en_US |
dc.subject.other | Adaptation to Growth in Axenic Culture | en_US |
dc.title | Method for the Simultaneous Establishment of Many Axenic Cultures of Paramecium * † | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Biological Sciences, The University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/74279/1/j.1550-7408.1978.tb03884.x.pdf | |
dc.identifier.doi | 10.1111/j.1550-7408.1978.tb03884.x | en_US |
dc.identifier.source | Journal of Eukaryotic Microbiology | en_US |
dc.identifier.citedreference | Allen SL, Gibson I. 1971. The purification of DNA from the genomes of Paramecium aurelia and Tetrahymena pyriformis. J. Protozool. 18, 518 – 25. | en_US |
dc.identifier.citedreference | Allen SL, Gibson I. 1971. Intersyngenic variations in the esterases of axenic stocks of Paramecium aurelia. Biochem. Genet. 5, 161 – 81. | en_US |
dc.identifier.citedreference | Allen SL, Farrow SW, Golembiewski PA. 1973. Esterase variations between the 14 syngens of Paramecium aurelia under axenic growth. Genetics 73, 561 – 73. | en_US |
dc.identifier.citedreference | Butzel HM, Horwitz H. 1965. Excystment of Didinium nasutum. J. Protozool. 12, 413 – 6. | en_US |
dc.identifier.citedreference | Gibson I, Cavill A. 1973. Effects of bacterial products on a Paramecium esterase. Biochem. Genet. 8, 357 – 64. | en_US |
dc.identifier.citedreference | Rowe E, Gibson I, Cavill A. 1971. The effects of growth conditions on the esterases of Paramecium aurelia. Biochem. Genet. 5, 151 – 9. | en_US |
dc.identifier.citedreference | Soldo AT, van Wagtendonk WJ. 1961. Nitrogen metabolism in Paramecium aurelia. J. Protozool. 8, 41 – 55. | en_US |
dc.identifier.citedreference | Soldo AT, Godoy GA, van Wagtendonk WJ. 1966. Growth of particle-bearing and particle-free Paramecium aurelia in axenic culture. J. Protozool. 13, 494 – 7. | en_US |
dc.identifier.citedreference | Sonneborn TM. 1950. Methods in the general biology and genetics of Paramecium aurelia. J. Exp. Zool. 113, 87 – 147. | en_US |
dc.identifier.citedreference | Sonneborn TM. 1957. Breeding systems, reproductive methods and species problems in protozoa, in Mayr E, ed., The Species Problem, Am. Assoc. Adv. Sci. Symp., Washington DC, pp. 155 – 324. | en_US |
dc.identifier.citedreference | Sonneborn TM. 1970. Methods in Paramecium research, in Prescott DM, ed., Methods Cell Physiol. 4, 241 – 339. | en_US |
dc.identifier.citedreference | Sonneborn TM. 1975. Paramecium aurelia, in King RC, ed., Handbook of Genetics, Plenum Press, New York, NY, 2, 469 – 594. | en_US |
dc.identifier.citedreference | Sonneborn TM. 1975. The Paramecium aurelia complex of fourteen sibling species. Trans. Am. Microsc. Soc. 94, 155 – 78. | en_US |
dc.identifier.citedreference | van Wagtendonk WJ. 1974. Nutrition of Paramecium, in van Wagtendonk WJ, ed., Paramecium—A Current Survey, Elsevier Sci. Pub. Co., New York, NY, pp. 339 – 376. | en_US |
dc.identifier.citedreference | van Wagtendonk WJ, Hackett PL. 1949. The culture of Paramecium aurelia in the absence of other living organisms. Proc. Nat. Acad. Sci. U.S. 35, 155 – 9. | en_US |
dc.identifier.citedreference | van Wagtendonk WJ, Soldo AT. 1970. Methods used in the axenic cultivation of Paramecium aurelia, in Prescott DM, ed., Methods Cell Physiol. 4, 117 – 30. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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