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Characterization of methanotrophic bacteria on the basis of intact phospholipid profiles
dc.contributor.authorFang, Jiasongen_US
dc.contributor.authorBarcelona, Michael J.en_US
dc.contributor.authorSemrau, Jeremy D.en_US
dc.date.accessioned2010-06-01T21:12:45Z
dc.date.available2010-06-01T21:12:45Z
dc.date.issued2000-08en_US
dc.identifier.citationFang, Jiasong; Barcelona, Michael J.; Semrau, Jeremy D. (2000). "Characterization of methanotrophic bacteria on the basis of intact phospholipid profiles." FEMS Microbiology Letters 189(1): 67-72. <http://hdl.handle.net/2027.42/74291>en_US
dc.identifier.issn0378-1097en_US
dc.identifier.issn1574-6968en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/74291
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10913867&dopt=citationen_US
dc.description.abstractThe intact phospholipid profiles (IPPs) of seven species of methanotrophs from all three physiological groups, type I, II and X, were determined using liquid chromatography/electrospray ionization/mass spectrometry. In these methanotrophs, two major classes of phospholipids were found, phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) as well as its derivatives phosphatidylmethylethanolamine (PME) and phosphatidyldimethylethanolamine (PDME). Specifically, the type I methanotrophs, Methylomonas methanica, Methylomonas rubra and Methylomicrobium album BG8 were characterized by PE and PG phospholipids with predominantly C16:1 fatty acids. The type II methanotrophs, Methylosinus trichosporium OB3b and CSC1 were characterized by phospholipids of PG, PME and PDME with predominantly C18:1 fatty acids. Methylococcus capsulatus Bath, a representative of type X methanotrophs, contained mostly PE (89% of the total phospholipids). Finally, the IPPs of a recently isolated acidophilic methanotroph, Methylocella palustris , showed it had a preponderance of PME phospholipids with 18:1 fatty acids (94% of total). Principal component analysis showed these methanotrophs could be clearly distinguished based on phospholipid profiles. Results from this study suggest that IPP can be very useful in bacterial chemotaxonomy.en_US
dc.format.extent263894 bytes
dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rights2000 Federation of European Microbiological Societiesen_US
dc.subject.otherPhospholipid Profileen_US
dc.subject.otherLC/ESI/MSen_US
dc.subject.otherMethanotrophen_US
dc.titleCharacterization of methanotrophic bacteria on the basis of intact phospholipid profilesen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Civil and Environmental Engineering, The University of Michigan, 1213 IST Bldg., 2200 Bonisteel Blvd., Ann Arbor, MI 48109, USAen_US
dc.contributor.affiliationumDepartment of Civil and Environmental Engineering, The University of Michigan, 181 EWRE Bldg., 1351 Beal Avenue, Ann Arbor, MI 48109, USAen_US
dc.identifier.pmid10913867en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/74291/1/j.1574-6968.2000.tb09207.x.pdf
dc.identifier.doi10.1111/j.1574-6968.2000.tb09207.xen_US
dc.identifier.sourceFEMS Microbiology Lettersen_US
dc.identifier.citedreferenceHanson, R.S., Hanson, T.E. ( 1996 ) Methanotrophic bacteria. Microbiol. Rev. 60, 439 – 471.en_US
dc.identifier.citedreference[2] Hanson, R.S. and Brusseau, G.A. (1994) Biodegradation of low-molecular-weight halogenated compounds by aerobic bacteria. In: Biological Degradation and Bioremediation of Toxic Cemicals (Chaudry, G.R., Ed.), pp. 227–297. Diocorides Press, Portland, OR.en_US
dc.identifier.citedreferenceLontoh, S., Semrau, J.D. ( 1998 ) Methane and trichloroethylene degradation by Methylosinus trichosporium OB3b expressing particulate methane monooxygenase. Appl. Environ. Microbiol. 64, 1106 – 1114.en_US
dc.identifier.citedreferencevan Hylckama Vleig, J.E.T., De Koning, W., Janssen, D.B. ( 1996 ) Transformation kinetics of chlorinated ethenes by Methylosinus trichosporium OB3b and detection of unstable epoxides by on-line gas chromatography. Appl. Environ. Microbiol. 62, 3304 – 3312.en_US
dc.identifier.citedreference[5] White, D.C., Bobbie, R.J., King, J.D., Nickels, J.S. and Amoe, P. (1979) Lipid analysis of sediments for microbial biomass and community structure. In: Methodology for Biomass Determination and Microbial Activities in Sediments (Litchfield, C.D. and Seyfried, P.L., Eds.), pp. 87–103. ASTM STP 673, American Society for Testing and Materials, Philadelphia, PA.en_US
dc.identifier.citedreferenceFang, J., Barcelona, M.J. ( 1998 ) Structural determination and quantitative analysis of phospholipids using liquid chromatography/electrospray ionization/mass spectrometry. J. Microbiol. Methods 33, 23 – 35.en_US
dc.identifier.citedreferenceBalkwill, D.L., Leach, F.R., Wilson, J.T., McNabb, J.F., White, D.C. ( 1988 ) Equivalence of microbial biomass measures based on membrane lipid and cell wall components, adenosine triphosphate and direct counts in subsurface aquifer sediments. Microb. Ecol. 16, 73 – 84.en_US
dc.identifier.citedreferenceWhittenbury, R.K., Philips, K.D., Wilkinson, J.F. ( 1970 ) Enrichment, isolation and some properties of methane-utilizing bacteria. J. Gen. Microbiol. 61, 205 – 218.en_US
dc.identifier.citedreferenceHenry, S.M., Grbic-Galic, D. ( 1991 ) Influence of endogenous and exogenous electron donors and trichloroethylene oxidation toxicity on trichloroethylene oxidation by methanotrophic cultures from a groundwater aquifer. Appl. Environ. Microbiol. 57, 236 – 244.en_US
dc.identifier.citedreferenceDedysh, S.N., Liesack, W., Khmelenina, V.N., Suzina, N.E., Trotsenko, Y.A., Semrau, J.D., Abing, A.M., Panikov, N.S., Tiedje, J.M. ( 1999 ) Methylocella palustris gen. nov., sp. nov., a new methane-oxidizing acidophilic bacterium from peat bogs representing a novel sub-type of serine pathway methanotrophs. Int. J. Syst. Bacteriol. 50, 955 – 969.en_US
dc.identifier.citedreferenceFang, J., Findlay, R.H. ( 1996 ) The use of a classic lipid extraction method for simultaneous recovery of organic pollutants and microbial lipids from sediments. J. Microbiol. Methods 27, 63 – 71.en_US
dc.identifier.citedreferenceLechevalier, M.P. ( 1977 ) Lipids in bacterial taxonomy – a taxonomist's view. Crit. Rev. Microbiol. 7, 109 – 210.en_US
dc.identifier.citedreferenceWeaver, T.L., Patrick, M.A., Dugan, P.R. ( 1975 ) Whole-cell and membrane lipids of the methylotrophic bacterium Methylosinus trichosporium. J. Bacteriol. 124, 602 – 605.en_US
dc.identifier.citedreference[14] Goldfine, H. (1972) Comparative aspects of bacterial lipids. In: Advances in Microbial Physiology (Rose, A.H. and Tempest, D.W., Eds.), pp. 1–58. Academic Press, London.en_US
dc.identifier.citedreferenceGoldberg, I., Jensen, A.P. ( 1975 ) Phospholipid and fatty acid composition of methanol-utilizing bacteria. J. Bacteriol. 130, 535 – 537.en_US
dc.identifier.citedreferenceMakula, R.A. ( 1978 ) Phospholipid composition of methane-utilizing bacteria. J. Bacteriol. 134, 771 – 777.en_US
dc.identifier.citedreferenceUrakami, T., Komagata, K. ( 1987 ) Cellular fatty acid composition with special reference to the existence of hydroxy fatty acids in Gram-negative methanol-, methane-, and methylamine-utilizing bacteria. J. Gen. Appl. Bacteriol. 33, 135 – 165.en_US
dc.identifier.citedreferenceBlumer, M., Chase, T., Watson, S.W. ( 1969 ) Fatty acids in the lipids of marine and terrestrial nitrifying bacteria. J. Bacteriol. 99, 366 – 370.en_US
dc.identifier.citedreferenceOelze, J., Drews, G. ( 1972 ) Membranes of photosynthetic bacteria. Biochim. Biophys. Acta 265, 209 – 239.en_US
dc.identifier.citedreferenceKaiser, H.F. ( 1960 ) The application of electronic computers to factor analysis. Educ. Psychol. Meas. 20, 141 – 151.en_US
dc.identifier.citedreferenceBowman, J.P., Sly, L.I., Nichols, P.D., Hyward, A.C. ( 1993 ) Revised taxonomy of the methanotrophs: Description of Methylobacter gen. nov., emendation of Methylococcus, validation of Methylosinus and Methylocystis species, and a proposal that the family Methylococcaceae includes only the group I methanotrophs. Int. J. Syst. Bacteriol. 43, 735 – 753.en_US
dc.identifier.citedreference[22] Fang, J., Barcelona, M.J. and Alveraz, P.J. (2000) A direct comparison between fatty acid analysis and intact phospholipid profiling for microbial identification, Org. Geochem., in press.en_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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