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Site-specific receptor methylation of FrzCD in Myxococcus xanthus is controlled by a tetra-trico peptide repeat (TPR) containing regulatory domain of the FrzF methyltransferase
dc.contributor.authorScott, Ansley E.en_US
dc.contributor.authorSimon, Eric S.en_US
dc.contributor.authorPark, Samuel K.en_US
dc.contributor.authorAndrews, Philipen_US
dc.contributor.authorZusman, David R.en_US
dc.date.accessioned2010-06-01T21:20:55Z
dc.date.available2010-06-01T21:20:55Z
dc.date.issued2008-08en_US
dc.identifier.citationScott, Ansley E.; Simon, Eric; Park, Samuel K.; Andrews, Philip; Zusman, David R. (2008). "Site-specific receptor methylation of FrzCD in Myxococcus xanthus is controlled by a tetra-trico peptide repeat (TPR) containing regulatory domain of the FrzF methyltransferase." Molecular Microbiology 69(3): 724-735. <http://hdl.handle.net/2027.42/74414>en_US
dc.identifier.issn0950-382Xen_US
dc.identifier.issn1365-2958en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/74414
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18554333&dopt=citationen_US
dc.description.abstractMyxococcus xanthus is a gliding bacterium with a complex life cycle that includes swarming, predation and fruiting body formation. Directed movements in M. xanthus are regulated by the Frz chemosensory system, which controls cell reversals. The Frz pathway requires the activity of FrzCD, a cytoplasmic methyl-accepting chemotaxis protein, and FrzF, a methyltransferase (CheR) containing an additional domain with three tetra trico-peptide repeats (TPRs). To investigate the role of the TPRs in FrzCD methylation, we used full-length FrzF and FrzF lacking its TPRs (FrzF CheR ) to methylate FrzCD in vitro . FrzF methylated FrzCD on a single residue, E182, while FrzF CheR methylated FrzCD on three residues, E168, E175 and E182, indicating that the TPRs regulate site-specific methylation. E168 and E182 were predicted consensus methylation sites, but E175 is methylated on an HE pair. To determine the roles of these sites in vivo , we substituted each methylatable glutamate with either an aspartate or an alanine residue and determined the impact of the point mutants on single cell reversals, swarming and fruiting body formation. Single, double and triple methylation site mutants revealed that each site played a unique role in M. xanthus behaviour and that the pattern of receptor methylation determined receptor activity. This work also shows that methylation can both activate and inactivate the receptor.en_US
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dc.publisherBlackwell Publishing Ltden_US
dc.rightsJournal compilation © 2008 Blackwell Publishingen_US
dc.titleSite-specific receptor methylation of FrzCD in Myxococcus xanthus is controlled by a tetra-trico peptide repeat (TPR) containing regulatory domain of the FrzF methyltransferaseen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan, Ann Arbor, MI, USAen_US
dc.contributor.affiliationotherDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.en_US
dc.identifier.pmid18554333en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/74414/1/MMI_6323_sm_Table_S1_and_Figures_S1-S5.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/74414/2/j.1365-2958.2008.06323.x.pdf
dc.identifier.doi10.1111/j.1365-2958.2008.06323.xen_US
dc.identifier.sourceMolecular Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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