In Vivo Conditioning of Tissue-engineered Heart Muscle Improves Contractile Performance
dc.contributor.author | Birla, Ravi K. | en_US |
dc.contributor.author | Borschel, Gregory H. | en_US |
dc.contributor.author | Dennis, Robert G. | en_US |
dc.date.accessioned | 2010-06-01T21:35:59Z | |
dc.date.available | 2010-06-01T21:35:59Z | |
dc.date.issued | 2005-11 | en_US |
dc.identifier.citation | Birla, Ravi K.; Borschel, Gregory H.; Dennis, Robert G. (2005). "In Vivo Conditioning of Tissue-engineered Heart Muscle Improves Contractile Performance." Artificial Organs 29(11): 866-875. <http://hdl.handle.net/2027.42/74650> | en_US |
dc.identifier.issn | 0160-564X | en_US |
dc.identifier.issn | 1525-1594 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/74650 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16266299&dopt=citation | en_US |
dc.description.abstract | The ability to engineer cardiac tissue in vitro is limited by the absence of a vasculature. In this study we describe an in vivo model which allows neovascularization of engineered cardiac tissue. Three-dimensional cardiac tissue, termed “cardioids,” was engineered in vitro from the spontaneous delamination of a confluent monolayer of cardiac cells. Cardioids were sutured onto a support framework and then implanted in a subcutaneous pocket in syngeneic recipient rats. Three weeks after implantation, cardioids were recovered for in vitro force testing and histological evaluation. Staining for hematoxylin and eosin demonstrated the presence of viable cells within explanted cardioids. Immunostaining with von Willebrand factor showed the presence of vascularization. Electron micrographs revealed the presence of large amounts of aligned contractile proteins and a high degree of intercellular connectivity. The peak active force increased from an average value of 57 µN for control cardioids to 447 µN for explanted cardioids. There was also a significant increase in the specific force. There was a significant decrease in the time to peak tension and half relaxation time. Explanted cardioids could be electrically paced at frequencies of 1–5 Hz. Explanted cardioids exhibited a sigmoidal response to calcium and positive chronotropy in response to epinephrine. As the field of cardiac tissue engineering progresses, it becomes desirable to engineer larger diameter tissue equivalents and to induce angiogenesis within tissue constructs. This study describes a relatively simple in vivo model, which promotes the neovascularization of tissue-engineered heart muscle and subsequent improvement in contractile performance. | en_US |
dc.format.extent | 465417 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Science Inc | en_US |
dc.rights | 2005 International Center for Artificial Organs and Transplantation | en_US |
dc.subject.other | Cell Culture | en_US |
dc.subject.other | Myocytes | en_US |
dc.subject.other | Contractile Function | en_US |
dc.subject.other | Tissue Engineering | en_US |
dc.subject.other | Angiogenesis | en_US |
dc.subject.other | Epinephrine | en_US |
dc.title | In Vivo Conditioning of Tissue-engineered Heart Muscle Improves Contractile Performance | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Medicine (General) | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | † Plastic and Reconstructive Surgery, The University of Michigan, Ann Arbor, MI; and | en_US |
dc.contributor.affiliationother | * Sections of Cardiac Surgery and | en_US |
dc.contributor.affiliationother | † Department of Biomedical Engineering, University of North Carolina, Chapel Hill, NC, U.S.A. | en_US |
dc.identifier.pmid | 16266299 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/74650/1/j.1525-1594.2005.00148.x.pdf | |
dc.identifier.doi | 10.1111/j.1525-1594.2005.00148.x | en_US |
dc.identifier.source | Artificial Organs | en_US |
dc.identifier.citedreference | 1. 2002 Heart and Stroke Statistical Update, American Heart Association. 2002. Available at: http://www.americanheart.org. | en_US |
dc.identifier.citedreference | Shimizu T, Yamato M, Kikuchi A, Okano T. Two-dimensional manipulation of cardiac myocyte sheets utilizing temperature-responsive culture dishes augments the pulsatile amplitude. Tissue Eng 2001; 7: 141 – 51. | en_US |
dc.identifier.citedreference | Shimizu T, Yamato M, Akutsu T, et al. Electrically communicating three-dimensional cardiac tissue mimic fabricated by layered cultured cardiomyocyte sheets. J Biomed Mater Res 2002; 60: 110 – 7. | en_US |
dc.identifier.citedreference | Shimizu T, Yamato M, Isoi Y, et al. Fabrication of pulsatile cardiac tissue grafts using a novel 3-dimensional cell sheet manipulation technique and temperature-responsive cell culture surfaces. Circ Res 2002; 90: e40. | en_US |
dc.identifier.citedreference | Bursac N, Papadaki M, Cohen RJ, et al. Cardiac muscle tissue engineering: toward an in vitro model for electrophysiological studies. Am J Physiol 1999; 277: t – 44. | en_US |
dc.identifier.citedreference | Carrier RL, Papadaki M, Rupnick M, et al. Cardiac tissue engineering: cell seeding, cultivation parameters, and tissue construct characterization. Biotechnol Bioeng 1999; 64: 580 – 9. | en_US |
dc.identifier.citedreference | Papadaki M, Bursac N, Langer R, Merok J, Vunjak-Novakovic G, Freed LE. Tissue engineering of functional cardiac muscle: molecular, structural, and electrophysiological studies. Am J Physiol-Heart C 2001; 280: H168 – 78. | en_US |
dc.identifier.citedreference | Li RK, Jia ZQ, Weisel RD, Mickle DA, Choi A, Yau TM. Survival and function of bioengineered cardiac grafts. Circulation 1999; 100 ( Suppl. ): 9. | en_US |
dc.identifier.citedreference | Li RK, Yau TM, Weisel RD, et al. Construction of a bioengineered cardiac graft. J Thorac Cardiovasc Surg 2000; 119: 368 – 75. | en_US |
dc.identifier.citedreference | Sakai T, Li RK, Weisel RD, et al. The fate of a tissue-engineered cardiac graft in the right ventricular outflow tract of the rat. J Thorac Cardiovasc Surg 2001; 121: 932 – 42. | en_US |
dc.identifier.citedreference | Leor J, Aboulafia-Etzion S, Dar A, et al. Bioengineered cardiac grafts: a new approach to repair the infarcted myocardium? Circulation 2000; 102 ( Suppl. ): 61. | en_US |
dc.identifier.citedreference | Eschenhagen T, Fink C, Remmers U, et al. Three-dimensional reconstitution of embryonic cardiomyocytes in a collagen matrix: a new heart muscle model system. FASEB J 1997; 11: 683 – 94. | en_US |
dc.identifier.citedreference | Souren JE, Schneijdenberg C, Verkleij AJ, Van Wijk R. Factors controlling the rhythmic contraction of collagen gels by neonatal heart cells. In Vitro Cell Dev B 1992; 28A: t – 204. | en_US |
dc.identifier.citedreference | Zimmermann WH, Fink C, Kralisch D, Remmers U, Weil J, Eschenhagen T. Three-dimensional engineered heart tissue from neonatal rat cardiac myocytes. Biotechnol Bioeng 2000; 68: 106 – 14. | en_US |
dc.identifier.citedreference | Zimmermann WH, Schneiderbanger K, Schubert P, et al. Tissue engineering of a differentiated cardiac muscle construct. [see comments.]. Circ Res 2002; 90: 223 – 30. | en_US |
dc.identifier.citedreference | Baar K, Birla R, Boluyt MO, Borschel GH, Arruda EM, Dennis RG. Heart muscle by design: self-organization of rat cardiac cells into contractile 3-D cardiac tissue. FASEB J 2005; 19: 275 – 7. | en_US |
dc.identifier.citedreference | Eschenhagen T, Didie M, Munzel F, Schubert P, Schneiderbanger K, Zimmermann WH. 3D engineered heart tissue for replacement therapy. Basic Res Cardiol 2002; 97 ( Suppl. ): 52. | en_US |
dc.identifier.citedreference | Boluyt MO, Zheng JS, Younes A, et al. Rapamycin inhibits alpha 1-adrenergic receptor-stimulated cardiac myocyte hypertrophy but not activation of hypertrophy-associated genes. Evidence for involvement of p70, S6 kinase. Circ Res 1997; 81: 176 – 86. | en_US |
dc.identifier.citedreference | Dennis RG, Kosnik PE, Gilbert ME, Faulkner JA. Excitability and contractility of skeletal muscle engineered from primary cultures and cell lines. Am J Physiol-Cell Ph 2001; 280: C288 – 95. | en_US |
dc.identifier.citedreference | Layland J, Young IS, Altringham JD. The effect of cycle frequency on the power output of rat papillary muscles in vitro. J Exp Biol 1995; 198: 1035 – 43. | en_US |
dc.identifier.citedreference | Friedman WF. The intrinsic physiologic properties of the developing heart. Prog Cardiovasc Dis 1972; 15: 87 – 111. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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