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Bacterial-associated cholera toxin and GM 1 binding are required for transcytosis of classical biotype Vibrio cholerae through an in vitro M cell model system
dc.contributor.authorBlanco, Luz P.en_US
dc.contributor.authorDiRita, Victor J.en_US
dc.date.accessioned2010-06-01T21:55:36Z
dc.date.available2010-06-01T21:55:36Z
dc.date.issued2006-06en_US
dc.identifier.citationBlanco, Luz P.; DiRita, Victor J. (2006). "Bacterial-associated cholera toxin and GM 1 binding are required for transcytosis of classical biotype Vibrio cholerae through an in vitro M cell model system." Cellular Microbiology 8(6): 982-998. <http://hdl.handle.net/2027.42/74960>en_US
dc.identifier.issn1462-5814en_US
dc.identifier.issn1462-5822en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/74960
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16681839&dopt=citationen_US
dc.description.abstractTo elucidate mechanisms involved in M cell uptake and transcytosis of Vibrio cholerae , we used an in vitro model of human M-like cells in a Caco-2 monolayer. Interspersed among the epithelial monolayer of Caco-2 cells we detect cells that display M-like features with or without prior lymphocyte treatment and we have established key parameters for V. cholerae transcytosis in this model. Cholera toxin (CT) mutants lacking the A subunit alone or both the A and B subunits were deficient for transcytosis. We explored this finding further and showed that expression of both subunits is required for binding by whole V. cholerae to immobilized CT receptor, the glycosphingolipid GM 1 . Confocal microscopy showed CT associated with transcytosing bacteria, and transcytosis was inhibited by pre-incubation with GM 1 before infection. Finally, heat treatment of the bacterial cells caused a loss of binding to GM 1 that was correlated with a significant decrease in uptake and transcytosis by the monolayer. Our data support a model in which the ability of bacteria to interact with GM 1 in a CT-dependent fashion plays a critical role in transcytosis of V. cholerae by M cells.en_US
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dc.publisherBlackwell Publishing Ltden_US
dc.rights© 2006 The Authors; Journal compilation © 2006 Blackwell Publishing Ltden_US
dc.titleBacterial-associated cholera toxin and GM 1 binding are required for transcytosis of classical biotype Vibrio cholerae through an in vitro M cell model systemen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA.en_US
dc.contributor.affiliationotherUnit for Laboratory Animal Medicine, anden_US
dc.identifier.pmid16681839en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/74960/1/j.1462-5822.2005.00681.x.pdf
dc.identifier.doi10.1111/j.1462-5822.2005.00681.xen_US
dc.identifier.sourceCellular Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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