cAMP and PMA enhance the effects of IGF-I in the proliferation of endometrial adenocarcinoma cell line HEC-1-A by acting at the G 1 phase of the cell cycle
dc.contributor.author | Talavera, Francisco | en_US |
dc.contributor.author | Bergman, C. | en_US |
dc.contributor.author | Pearl, Michael L. | en_US |
dc.contributor.author | Connor, Patrick | en_US |
dc.contributor.author | Roberts, James A. | en_US |
dc.contributor.author | Menon, K. M. J. | en_US |
dc.date.accessioned | 2010-06-01T21:58:57Z | |
dc.date.available | 2010-06-01T21:58:57Z | |
dc.date.issued | 1995-03 | en_US |
dc.identifier.citation | Talavera, F.; Bergman, C.; Pearl, M. L.; Connor, P.; Roberts, J. A.; Menon, K. M. J. (1995). "cAMP and PMA enhance the effects of IGF-I in the proliferation of endometrial adenocarcinoma cell line HEC-1-A by acting at the G 1 phase of the cell cycle." Cell Proliferation 28(3): 121-136. <http://hdl.handle.net/2027.42/75013> | en_US |
dc.identifier.issn | 0960-7722 | en_US |
dc.identifier.issn | 1365-2184 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/75013 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7734622&dopt=citation | en_US |
dc.description.abstract | The present study was undertaken to determine whether endometrial cancer cell line HEC-1-A differ from nontransformed cells, in that the cAMP and protein kinase C pathways may enhance IGF-I effects in mitogenesis by acting at the G 1 phase of the cell cycle instead of G 0 . Immunofluorescence staining of HEC-1-A cells using the proliferating cell nuclear antigen (PCNA) monoclonal antibody and flow cytometric analysis determined that HEC-1-A cells do not enter the G 0 phase of the cell cycle when incubated in a serum-free medium. Approximately 51% of the cells were in G 1 , 12% were in S and 37% in G 2 phase of the cell cycle prior to treatment. Forskolin and phorbol-12-myristate 13-acetate (PMA) were used to stimulate cAMP production and protein kinase C activity, respectively. IGF-I, forskolin and PMA each increased ( P <0.01) [ 3 H]-thymidine incorporation in a dose and time dependent manner. The interaction of forskolin and PMA with IGF-I was then determined. Cells preincubated with forskolin or PMA followed by incubation with IFG-I incorporated significantly more ( P <0.01) [ 3 H]-thymidine into DNA than controls or any treatment alone. It is concluded that forskolin and, to a lesser extent, PMA exert their effect at the G 1 phase of the cycle to enhance IGF-I effects in cell proliferation. | en_US |
dc.format.extent | 1418828 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | 1995 Blackwell Science Limited | en_US |
dc.title | cAMP and PMA enhance the effects of IGF-I in the proliferation of endometrial adenocarcinoma cell line HEC-1-A by acting at the G 1 phase of the cell cycle | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Obstetrics and Gynecology, University of Michigan Medical Center, Ann Arbor, Michigan, USA | en_US |
dc.identifier.pmid | 7734622 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/75013/1/j.1365-2184.1995.tb00061.x.pdf | |
dc.identifier.doi | 10.1111/j.1365-2184.1995.tb00061.x | en_US |
dc.identifier.source | Cell Proliferation | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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