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Single amino acid substitutions in either YhjD or MsbA confer viability to 3-deoxy-d- manno -oct-2-ulosonic acid-depleted Escherichia coli
dc.contributor.authorMamat, Uween_US
dc.contributor.authorMeredith, Timothy C.en_US
dc.contributor.authorAggarwal, Paragen_US
dc.contributor.authorKühl, Annikaen_US
dc.contributor.authorKirchhoff, Paulen_US
dc.contributor.authorLindner, Bukoen_US
dc.contributor.authorHanuszkiewicz, Annaen_US
dc.contributor.authorSun, Jenniferen_US
dc.contributor.authorHolst, Ottoen_US
dc.contributor.authorWoodard, Ronald W.en_US
dc.date.accessioned2010-06-01T22:06:10Z
dc.date.available2010-06-01T22:06:10Z
dc.date.issued2008-02en_US
dc.identifier.citationMamat, Uwe; Meredith, Timothy C.; Aggarwal, Parag; KÜhl, Annika; Kirchhoff, Paul; Lindner, Buko; Hanuszkiewicz, Anna; Sun, Jennifer; Holst, Otto; Woodard, Ronald W. (2008). "Single amino acid substitutions in either YhjD or MsbA confer viability to 3-deoxy-d- manno -oct-2-ulosonic acid-depleted Escherichia coli ." Molecular Microbiology 67(3): 633-648. <http://hdl.handle.net/2027.42/75126>en_US
dc.identifier.issn0950-382Xen_US
dc.identifier.issn1365-2958en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/75126
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18093093&dopt=citationen_US
dc.description.abstractThe Escherichia coli K-12 strain KPM22, defective in synthesis of 3-deoxy-d- manno -oct-2-ulosonic acid (Kdo), is viable with an outer membrane (OM) composed predominantly of lipid IV A , a precursor of lipopolysaccharide (LPS) biosynthesis that lacks any glycosylation. To sustain viability, the presence of a second-site suppressor was proposed for transport of lipid IV A from the inner membrane (IM), thus relieving toxic side-effects of lipid IV A accumulation and providing sufficient amounts of LPS precursors to support OM biogenesis. We now report the identification of an arginine to cysteine substitution at position 134 of the conserved IM protein YhjD in KPM22 that acts as a compensatory suppressor mutation of the lethal δKdo phenotype. Further, the yhjD400 suppressor allele renders the LPS transporter MsbA dispensable for lipid IV A transmembrane trafficking. The independent derivation of a series of non-conditional KPM22-like mutants from the Kdo-dependent parent strain TCM15 revealed a second class of suppressor mutations localized to MsbA. Proline to serine substitutions at either residue 18 or 50 of MsbA relieved the Kdo growth dependence observed in the isogenic wild-type strain. The possible impact of these suppressor mutations on structure and function are discussed by means of a computationally derived threading model of MsbA.en_US
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dc.publisherBlackwell Publishing Ltden_US
dc.rights© 2007 The Authors; Journal compilation © 2007 Blackwell Publishing Ltden_US
dc.titleSingle amino acid substitutions in either YhjD or MsbA confer viability to 3-deoxy-d- manno -oct-2-ulosonic acid-depleted Escherichia colien_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Medicinal Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.en_US
dc.contributor.affiliationotherDivisions of Structural Biochemistry anden_US
dc.contributor.affiliationotherImmunochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, D-23845 Borstel, Germany.en_US
dc.identifier.pmid18093093en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/75126/1/MMI_6074_sm_Figure_S1.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/75126/2/j.1365-2958.2007.06074.x.pdf
dc.identifier.doi10.1111/j.1365-2958.2007.06074.xen_US
dc.identifier.sourceMolecular Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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