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Enhancement of CD8 T-cell function through modifying surface glycoproteins in young and old mice
dc.contributor.authorBerger, Scott B.en_US
dc.contributor.authorMiller, Richard A.en_US
dc.contributor.authorSadighi Akha, Amir A.en_US
dc.date.accessioned2010-06-01T22:10:48Z
dc.date.available2010-06-01T22:10:48Z
dc.date.issued2006-10en_US
dc.identifier.citationSadighi Akha, Amir A.; Berger, Scott B.; Miller, Richard A. (2006). "Enhancement of CD8 T-cell function through modifying surface glycoproteins in young and old mice." Immunology 119(2): 187-194. <http://hdl.handle.net/2027.42/75199>en_US
dc.identifier.issn0019-2805en_US
dc.identifier.issn1365-2567en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/75199
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16805788&dopt=citationen_US
dc.description.abstractPrevious work from our laboratory has shown that modifying cell surface glycosylation with either a Clostridium perfringens -derived sialidase (CP-Siase), or an O-linked glycoprotein endopeptidase (OSGE) can enhance the function of CD4 T cells from both young and old mice at multiple levels. Here we have re-assessed the effect of age on CD8 T-cell function, and examined the outcome of enzymatic treatment with CP-Siase and OSGE on its different aspects. Pre-treatment of CD8 T cells with either CP-Siase or OSGE led to a significant increase in anti-CD3-mediated Ca 2+ response in both young and old mice. Pre-treated CD8 T cells from both age groups also displayed a significant increase in activation-induced CD69 and CD25 expression, and produced significantly higher amounts of interleukin-2 and interferon-γ in comparison to their untreated counterparts. Furthermore, pretreatment with either enzyme enhanced granzyme B expression in CD8 T cells, and increased their cytolytic activity in vitro . These data support the notion that glycosylated surface proteins hinder CD8 T-cell activation and function in both young and old mice, and raise the possibility of significantly improving CD8 T cell function in older individuals through enzymatic alteration of surface glycoproteins.en_US
dc.format.extent785405 bytes
dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rights2006 Blackwell Publishing Ltden_US
dc.subject.otherRodenten_US
dc.subject.otherAgeingen_US
dc.subject.otherTCRen_US
dc.subject.otherSignal Transductionen_US
dc.subject.otherGlycosylationen_US
dc.titleEnhancement of CD8 T-cell function through modifying surface glycoproteins in young and old miceen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumthe Geriatrics Center, University of Michigan Medical School, Ann Arbor, MI, USAen_US
dc.contributor.affiliationotherPathologyen_US
dc.contributor.affiliationotherBiological Chemistryen_US
dc.contributor.affiliationotherAnn Arbor DVA Medical Center, Ann Arbor, MI, USAen_US
dc.identifier.pmid16805788en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/75199/1/j.1365-2567.2006.02420.x.pdf
dc.identifier.doi10.1111/j.1365-2567.2006.02420.xen_US
dc.identifier.sourceImmunologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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