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Governor of the glnAp2 promoter of Escherichia coli
dc.contributor.authorAtkinson, Mariette R.en_US
dc.contributor.authorPattaramanon, Narinpornen_US
dc.contributor.authorNinfa, Alexander J.en_US
dc.date.accessioned2010-06-01T22:24:34Z
dc.date.available2010-06-01T22:24:34Z
dc.date.issued2002-12en_US
dc.identifier.citationAtkinson, Mariette R.; Pattaramanon, Narinporn; Ninfa, Alexander J. (2002). "Governor of the glnAp2 promoter of Escherichia coli ." Molecular Microbiology 46(5): 1247-1257. <http://hdl.handle.net/2027.42/75402>en_US
dc.identifier.issn0950-382Xen_US
dc.identifier.issn1365-2958en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/75402
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=12453212&dopt=citationen_US
dc.description.abstractLow-affinity sites for the activator NRI∼P (NtrC∼P) that map between the enhancer and the glnAp2 promoter were responsible for limiting promoter activity at high concentrations of NRI∼P in intact cells and in an in vitro transcription system consisting of purified bacterial components. That is, the low-affinity sites constitute a ‘governor’, limiting the maximum promoter activity. As the governor sites are themselves far from the promoter, they apparently act either by preventing the formation of the activation DNA loop that brings the enhancer-bound activator and the promoter-bound polymerase into proximity or by preventing a productive interaction between the enhancer-bound activator and polymerase. The combination of potent enhancer and governor sites at the glnAp2 promoter provides for efficient activation of the promoter when the activator concentration is low, while limiting the maximum level of promoter activity when the activator concentration is high.en_US
dc.format.extent237095 bytes
dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Science Ltden_US
dc.rightsBlackwell Science, 2002en_US
dc.titleGovernor of the glnAp2 promoter of Escherichia colien_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109-0606, USA.en_US
dc.identifier.pmid12453212en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/75402/1/j.1365-2958.2002.03211.x.pdf
dc.identifier.doi10.1046/j.1365-2958.2002.03211.xen_US
dc.identifier.sourceMolecular Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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