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Endocytosis of plasma-derived factor V by megakaryocytes occurs via a clathrin-dependent, specific membrane binding event
dc.contributor.authorBouchard, B. A.en_US
dc.contributor.authorWilliams, J. L.en_US
dc.contributor.authorMeisler, N. T.en_US
dc.contributor.authorLong, Michael W.en_US
dc.contributor.authorTracy, P. B.en_US
dc.date.accessioned2010-06-01T22:29:06Z
dc.date.available2010-06-01T22:29:06Z
dc.date.issued2005-03en_US
dc.identifier.citationBOUCHARD, B. A.; WILLIAMS, J. L.; MEISLER, N. T.; LONG, M. W.; TRACY, P. B. (2005). "Endocytosis of plasma-derived factor V by megakaryocytes occurs via a clathrin-dependent, specific membrane binding event." Journal of Thrombosis and Haemostasis 3(3): 541-551. <http://hdl.handle.net/2027.42/75473>en_US
dc.identifier.issn1538-7933en_US
dc.identifier.issn1538-7836en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/75473
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=15748245&dopt=citationen_US
dc.description.abstractMegakaryocytes were analyzed for their ability to endocytose factor V to define the cellular mechanisms regulating this process. In contrast to fibrinogen, factor V was endocytosed by megakaryocytes derived from CD34 + cells or megakaryocyte-like cell lines, but not by platelets. CD41 + ex vivo -derived megakaryocytes endocytosed factor V, as did subpopulations of the megakaryocyte-like cells MEG-01, and CMK. Similar observations were made for fibrinogen. Phorbol diester-induced megakaryocytic differentiation of the cell lines resulted in a substantial increase in endocytosis of both proteins as compared to untreated cells that did not merely reflect their disparate plasma concentrations. Factor IX, which does not associate with platelets or megakaryocytes, was not endocytosed by any of the cells examined. Endocytosis of factor V by megakaryocytes proceeds through a specific and independent mechanism as CHRF-288 cells endocytosed fibrinogen but not factor V, and the presence of other plasma proteins had no effect on the endocytosis of factor V by MEG-01 cells. Furthermore, as the endocytosis of factor V was also demonstrated to occur through a clathrin-dependent mechanism, these combined data demonstrate that endocytosis of factor V by megakaryocytes occurs via a specific, independent, and most probably receptor-mediated, event.en_US
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dc.format.extent3109 bytes
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dc.publisherBlackwell Science Incen_US
dc.rights2005 International Society on Thrombosis and Haemostasisen_US
dc.subject.otherClathrinen_US
dc.subject.otherEndocytosisen_US
dc.subject.otherFactor Ven_US
dc.subject.otherMegakaryocyteen_US
dc.subject.otherPlateleten_US
dc.subject.otherReceptoren_US
dc.titleEndocytosis of plasma-derived factor V by megakaryocytes occurs via a clathrin-dependent, specific membrane binding eventen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelInternal Medicine and Specialtiesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationum† Department of Pediatrics, University of Michigan, Ann Arbor, MI, USAen_US
dc.contributor.affiliationother* Biochemistryen_US
dc.contributor.affiliationother† Medicine, University of Vermont College of Medicine, Burlington, VT, USAen_US
dc.identifier.pmid15748245en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/75473/1/j.1538-7836.2005.01190.x.pdf
dc.identifier.doi10.1111/j.1538-7836.2005.01190.xen_US
dc.identifier.sourceJournal of Thrombosis and Haemostasisen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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