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An algorithm for extracting the network geometry of three-dimensional collagen gels

dc.contributor.authorStein, Andrew M.en_US
dc.contributor.authorVader, David A.en_US
dc.contributor.authorJawerth, Louise M.en_US
dc.contributor.authorWeitz, David A.en_US
dc.contributor.authorSander, Leonard M.en_US
dc.date.accessioned2010-06-01T22:29:18Z
dc.date.available2010-06-01T22:29:18Z
dc.date.issued2008-12en_US
dc.identifier.citationSTEIN, ANDREW M.; VADER, DAVID A.; JAWERTH, LOUISE M.; WEITZ, DAVID A.; SANDER, LEONARD M. (2008). "An algorithm for extracting the network geometry of three-dimensional collagen gels." Journal of Microscopy 232(3): 463-475. <http://hdl.handle.net/2027.42/75476>en_US
dc.identifier.issn0022-2720en_US
dc.identifier.issn1365-2818en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/75476
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=19094023&dopt=citationen_US
dc.description.abstractThe geometric structure of a biopolymer network impacts its mechanical and biological properties. In this paper, we develop an algorithm for extracting the network architecture of three-dimensional (3d) fluorescently labeled collagen gels, building on the initial work of Wu et al., (2003) . Using artificially generated images, the network extraction algorithm is then validated for its ability to reconstruct the correct bulk properties of the network, including fiber length, persistence length, cross-link density, and shear modulus.en_US
dc.format.extent877507 bytes
dc.format.extent3109 bytes
dc.format.mimetypeapplication/pdf
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dc.publisherBlackwell Publishing Ltden_US
dc.rightsJournal compilation © 2008 Royal Microscopical Societyen_US
dc.subject.other3d Reconstructionen_US
dc.subject.otherBiopolymer Networken_US
dc.subject.otherCollagen Gelen_US
dc.subject.otherConfocal Microscopyen_US
dc.subject.otherImage Processingen_US
dc.titleAn algorithm for extracting the network geometry of three-dimensional collagen gelsen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelScience (General)en_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationum† Michigan Center for Theoretical Physics and Department of Physics, University of Michigan, Ann Arbor, Michigan 48109, USAen_US
dc.contributor.affiliationother* Institute for Mathematics and its Applications, University of Minnesota, Minneapolis, MN 55403, USA, + astein@ima.umn.edu +en_US
dc.contributor.affiliationother† School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USAen_US
dc.identifier.pmid19094023en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/75476/1/j.1365-2818.2008.02141.x.pdf
dc.identifier.doi10.1111/j.1365-2818.2008.02141.xen_US
dc.identifier.sourceJournal of Microscopyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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