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Identification and characterization of genes encoding sex pheromone cAM373 activity in Enterococcus faecalis and Staphylococcus aureus

dc.contributor.authorFlannagan, Susan E.en_US
dc.contributor.authorClewell, Don B.en_US
dc.date.accessioned2010-06-01T22:37:47Z
dc.date.available2010-06-01T22:37:47Z
dc.date.issued2002-05en_US
dc.identifier.citationFlannagan, Susan E . ; Clewell, Don B . (2002). "Identification and characterization of genes encoding sex pheromone cAM373 activity in Enterococcus faecalis and Staphylococcus aureus ." Molecular Microbiology 44(3): 803-817. <http://hdl.handle.net/2027.42/75605>en_US
dc.identifier.issn0950-382Xen_US
dc.identifier.issn1365-2958en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/75605
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=11994160&dopt=citationen_US
dc.description.abstractThe sex pheromone cAM373 of Enterococcus faecalis and the related staph -cAM373 of Staphylococcus aureus were found to correspond to heptapeptides located within the C-termini of the signal sequences of putative prelipoproteins. The deduced mature forms of the lipoproteins share no detectable homology and presumably serve unrelated functions in the cells. The chromosomally encoded genetic determinants for production of the pheromones have been identified and designated camE (encoding cAM373) and camS (encoding staph -cAM373). Truncated and full-length clones of camE were generated in Escherichia coli , in which cAM373 activity was expressed. In E. faecalis , insertional inactivation in the middle of camE had no detectable phenotypic effects on the pheromone system. Establishment of an in frame translation stop codon within the signal sequence resulted in reduction of cAM373 activity to 3% of normal levels. The camS determinant has homologues in Staphylococcus epidermidis , Bacillus subtilis and Listeria monocytogenes ; however, corresponding heptapeptides present within those sequences do not resemble staph -cAM373 closely. The particular significance of staph -cAM373 as a potential intergeneric inducer of transfer-proficient genetic elements is discussed.en_US
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dc.publisherBlackwell Science Ltd.en_US
dc.rights2002 Blackwell Science Ltd.en_US
dc.titleIdentification and characterization of genes encoding sex pheromone cAM373 activity in Enterococcus faecalis and Staphylococcus aureusen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Microbiology and Immunology, School of Medicine, The University of Michigan, Ann Arbor, MI 48109, USA.en_US
dc.identifier.pmid11994160en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/75605/1/j.1365-2958.2002.02922.x.pdf
dc.identifier.doi10.1046/j.1365-2958.2002.02922.xen_US
dc.identifier.sourceMolecular Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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