Helicobacter pylori -Pulsed Dendritic Cells Induce H. pylori -Specific Immunity in Mice
dc.contributor.author | Zhang, Min | en_US |
dc.contributor.author | Berndt, Bradford E. | en_US |
dc.contributor.author | Eaton, Kathryn A. | en_US |
dc.contributor.author | Rathinavelu, Sivaprakash | en_US |
dc.contributor.author | Pierzchala, Anna | en_US |
dc.contributor.author | Kao, John Y. | en_US |
dc.date.accessioned | 2010-06-01T22:44:35Z | |
dc.date.available | 2010-06-01T22:44:35Z | |
dc.date.issued | 2008-06 | en_US |
dc.identifier.citation | Zhang, Min; Berndt, Bradford E.; Eaton, Kathryn A.; Rathinavelu, Sivaprakash; Pierzchala, Anna; Kao, John Y. (2008). " Helicobacter pylori -Pulsed Dendritic Cells Induce H. pylori -Specific Immunity in Mice." Helicobacter 13(3): 200-208. <http://hdl.handle.net/2027.42/75711> | en_US |
dc.identifier.issn | 1083-4389 | en_US |
dc.identifier.issn | 1523-5378 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/75711 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=18466395&dopt=citation | en_US |
dc.description.abstract | Background: The growing concern over the emergence of antibiotic-resistant Helicobacter pylori infection is propelling the development of an efficacious vaccine to control this highly adaptive organism. Aim: We studied the use of a dendritic cell (DC)-based vaccine against H. pylori infection in mice. Methods: The cellular immune responses to murine bone marrow-derived DCs pulsed with phosphate-buffered saline (PBS-DC) or live H. pylori SS1 (HP-DC) were assessed in vitro and in vivo. The protective immunity against H. pylori SS1 oral challenge was compared between HP-DC or PBS-DC immunized mice. The effect of regulatory T-cell (Treg) depletion by anti-CD25 antibody on HP-DC vaccine efficacy was also evaluated. Results: HP-DC induced a Th1-dominant response in vitro. In vivo, HP-DC immunized mice were characterized by a mixed Th1/Th2 peripheral immune response. However, in the stomach, HP-DC immunized mice expressed a higher level of IFN-γ compared to PBS-DC immunized mice; no difference was found for interleukin-5 expressions in the stomach. A lower bacterial colonization post- H. pylori challenge was observed in HP-DC immunized mice compared to PBS-DC immunized mice with no significant difference in gastritis severity. H. pylori -specific Th1 response and protective immunity were further enhanced in vivo by depletion of Treg with anti-CD25 antibody. Conclusion: DC-based anti- H. pylori vaccine induced H. pylori -specific helper T-cell responses capable of limiting bacterial colonization. Our data support the critical role of effector cellular immune response in the development of H. pylori vaccine. | en_US |
dc.format.extent | 326816 bytes | |
dc.format.extent | 3109 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.rights | Journal compilation © 2008 Blackwell Publishing Ltd. | en_US |
dc.subject.other | Vaccine | en_US |
dc.subject.other | Dendritic Cells | en_US |
dc.subject.other | Th1/Th2 | en_US |
dc.subject.other | Regulatory T Cells | en_US |
dc.subject.other | CD25 Depletion | en_US |
dc.title | Helicobacter pylori -Pulsed Dendritic Cells Induce H. pylori -Specific Immunity in Mice | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan 48109, USA | en_US |
dc.contributor.affiliationother | Internal Medicine (Gastroenterology Division) and | en_US |
dc.identifier.pmid | 18466395 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/75711/1/j.1523-5378.2008.00606.x.pdf | |
dc.identifier.doi | 10.1111/j.1523-5378.2008.00606.x | en_US |
dc.identifier.source | Helicobacter | en_US |
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dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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