Intracellular trafficking and replication of Burkholderia cenocepacia in human cystic fibrosis airway epithelial cells

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dc.contributor.author Sajjan, Umadevi S. en_US
dc.contributor.author Yang, Jeffrey H. en_US
dc.contributor.author Hershenson, Marc B. en_US
dc.contributor.author LiPuma, John J. en_US
dc.date.accessioned 2010-06-01T22:46:43Z
dc.date.available 2010-06-01T22:46:43Z
dc.date.issued 2006-09 en_US
dc.identifier.citation Sajjan, Umadevi S.; Yang, Jeffrey H.; Hershenson, Marc B.; LiPuma, John J. (2006). "Intracellular trafficking and replication of Burkholderia cenocepacia in human cystic fibrosis airway epithelial cells." Cellular Microbiology 8(9): 1456-1466. <http://hdl.handle.net/2027.42/75744> en_US
dc.identifier.issn 1462-5814 en_US
dc.identifier.issn 1462-5822 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/75744
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16922864&dopt=citation en_US
dc.description.abstract We investigated the trafficking of Burkholderia cenocepacia , an opportunistic respiratory pathogen of persons with cystic fibrosis (CF), in immortalized CF airway epithelial cells in vitro . Our results indicate that bacteria enter cells in a process involving actin rearrangement. Whereas both live and heat-killed bacteria reside transiently in early endosomes, only live bacteria escape from late endosomes to colocalize in vesicles positive for lysosomal membrane marker LAMP1, endoplasmic reticulum (ER) membrane marker calnexin, and autophagosome marker monodansylcadavarine (MDC). Twenty-four hours after infection, microcolonies of live bacteria were observed in the perinuclear area colocalizing with calnexin. In contrast, after ingestion, dead bacteria colocalized with late endosome marker Rab7, and lysosome markers LAMP1 and cathepsin D, but not with calnexin or MDC. Six to eight hours after ingestion of dead bacteria, degraded bacterial particles were observed in the cytoplasm and in vesicles positive for cathepsin D. These results indicate that live B. cenocepacia gain entry into human CF airway cells by endocytosis, escape from late endosomes to enter autophagosomes that fail to fuse with mature lysosomes, and undergo replication in the ER. This survival and replication strategy may contribute to the capacity of B. cenocepacia to persist in the lungs of infected CF patients. en_US
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dc.format.extent 3109 bytes
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dc.publisher Blackwell Publishing Ltd en_US
dc.rights © 2006 The Authors; Journal compilation © 2006 Blackwell Publishing Ltd en_US
dc.title Intracellular trafficking and replication of Burkholderia cenocepacia in human cystic fibrosis airway epithelial cells en_US
dc.type Article en_US
dc.subject.hlbsecondlevel "Molecular, Cellular and Developmental Biology" en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.identifier.pmid 16922864 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/75744/1/j.1462-5822.2006.00724.x.pdf
dc.identifier.doi 10.1111/j.1462-5822.2006.00724.x en_US
dc.identifier.source Cellular Microbiology en_US
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dc.owningcollname Interdisciplinary and Peer-Reviewed
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