Expression of BK-type calcium-activated potassium channel splice variants during chick cochlear development
dc.contributor.author | Kim, Jung-Min | en_US |
dc.contributor.author | Beyer, Ryan | en_US |
dc.contributor.author | Morales, Marti | en_US |
dc.contributor.author | Chen, Stephanie | en_US |
dc.contributor.author | Liu, Li Qian | en_US |
dc.contributor.author | Duncan, R. Keith | en_US |
dc.date.accessioned | 2010-06-02T19:46:17Z | |
dc.date.available | 2011-03-01T16:26:45Z | en_US |
dc.date.issued | 2010-07-01 | en_US |
dc.identifier.citation | Kim, Jung-Min; Beyer, Ryan; Morales, Marti; Chen, Stephanie; Liu, Li Qian; Duncan, R. Keith (2010). "Expression of BK-type calcium-activated potassium channel splice variants during chick cochlear development." The Journal of Comparative Neurology 518(13): 2554-2569. <http://hdl.handle.net/2027.42/75768> | en_US |
dc.identifier.issn | 0021-9967 | en_US |
dc.identifier.issn | 1096-9861 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/75768 | |
dc.description.abstract | The appearance of large-conductance, calcium-activated potassium (BK) current is a hallmark of functional maturation in auditory hair cells. Acquisition of this fast-activating current enables high-frequency, graded receptor potentials in all vertebrates and an electrical tuning mechanism in nonmammals. The gene encoding BK Α subunits is highly alternatively spliced, and the resulting variations in channel isoforms may contribute to functional diversity at the onset of hearing. We examined the tissue specificity of nine BK Α alternative exons and investigated changes in expression during chick cochlear development using quantitative polymerase chain reaction (qPCR). Each alternative was widely expressed in several tissues except for an insert near the C-terminus Ca 2+ sensing domain, which appeared brain-specific. The only alternative form in the membrane-bound core of the channel was expressed in brain and muscle but was undetected in cochlea. Of the remaining variants, three increased in expression prior to the onset of hearing and acquisition of BK currents. These three variants cause decreased Ca 2+ sensitivity or increased intracellular retention, traits that would not easily explain the advent of calcium-sensitive currents at embryonic day (E)18–19. Expression levels of other variants were mature and stable by E15, days before currents were acquired. Surface expression of C-terminal isoforms was examined using patch-clamp electrophysiology and immunocytochemistry. C-terminal variants that exhibit robust surface expression appeared in the membrane at E18, even though transcripts were unchanged during development starting from E12. These results indicate that delays in protein synthesis and trafficking/scaffolding of channel subunits underlie the late acquisition of BK currents in cochlear hair cells. J. Comp. Neurol. 518:2554–2569, 2010. © 2010 Wiley-Liss, Inc. | en_US |
dc.format.extent | 622912 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Neuroscience, Neurology and Psychiatry | en_US |
dc.title | Expression of BK-type calcium-activated potassium channel splice variants during chick cochlear development | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Kresge Hearing Research Institute, University of Michigan, Ann Arbor, Michigan 48109 ; Medical Sciences, Boston University School of Medicine, Boston, Massachusetts 02215 ; The first two authors contributed equally. | en_US |
dc.contributor.affiliationum | Kresge Hearing Research Institute, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Kresge Hearing Research Institute, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Kresge Hearing Research Institute, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Kresge Hearing Research Institute, University of Michigan, Ann Arbor, Michigan 48109 | en_US |
dc.contributor.affiliationum | Kresge Hearing Research Institute, University of Michigan, Ann Arbor, Michigan 48109 ; Kresge Hearing Research Institute, University of Michigan, 5323A Medical Science Building I, 1150 W. Medical Center Drive, Ann Arbor, MI 48109-5616 | en_US |
dc.identifier.pmid | 20503427 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/75768/1/22352_ftp.pdf | |
dc.identifier.doi | 10.1002/cne.22352 | en_US |
dc.identifier.source | The Journal of Comparative Neurology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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