Characterization of the chemotactic and mitogenic response of SMCs to PDGF-BB and FGF-2 in fibrin hydrogels
dc.contributor.author | Ucuzian, Areck A. | en_US |
dc.contributor.author | Brewster, Luke P. | en_US |
dc.contributor.author | East, Andrea T. | en_US |
dc.contributor.author | Pang, Yongang | en_US |
dc.contributor.author | Gassman, Andrew A. | en_US |
dc.contributor.author | Greisler, Howard P. | en_US |
dc.date.accessioned | 2010-08-02T17:49:06Z | |
dc.date.available | 2011-03-01T16:26:43Z | en_US |
dc.date.issued | 2010-09-01 | en_US |
dc.identifier.citation | Ucuzian, Areck A.; Brewster, Luke P.; East, Andrea T.; Pang, Yongang; Gassman, Andrew A.; Greisler, Howard P. (2010). "Characterization of the chemotactic and mitogenic response of SMCs to PDGF-BB and FGF-2 in fibrin hydrogels." Journal of Biomedical Materials Research Part A 94A(3): 988-996. <http://hdl.handle.net/2027.42/77533> | en_US |
dc.identifier.issn | 1549-3296 | en_US |
dc.identifier.issn | 1552-4965 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/77533 | |
dc.description.abstract | The delivery of growth factors to cellularize biocompatible scaffolds like fibrin is a commonly used strategy in tissue engineering. We characterized smooth muscle cells (SMC) proliferation and chemotaxis in response to PDGF-BB and FGF-2, alone and in combination, in 2D culture and in 3D fibrin hydrogels. While both growth factors induced an equipotent mitogenic response in 2D culture, only FGF-2 was significantly mitogenic for SMCs in 3D culture. Only PDGF-BB was significantly chemotactic in a modified Boyden chamber assay. In a 3D assay of matrix invasion, both growth factors induced an invasive response into the fibrin hydrogel in both proliferating and nonproliferating, mitomycin C (MMC) treated cells. The invasive response was less attenuated by the inhibition of proliferation in PDGF-BB stimulated cells compared with FGF-2 stimulated cells. We conclude that SMCs cultured in fibrin hydrogels have a more robust chemotactic response to PDGF-BB compared with FGF-2, and that the response to FGF-2 is more dependent on cell proliferation. Delivery of both growth factors together potentiates the chemotactic, but not mitogenic response to either growth factor alone. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010 | en_US |
dc.format.extent | 639775 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer and Materials Science | en_US |
dc.title | Characterization of the chemotactic and mitogenic response of SMCs to PDGF-BB and FGF-2 in fibrin hydrogels | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biomedical Engineering | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Surgery, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | Department of Surgery, Loyola University Medical Center, Maywood, Illinois ; Department of Cell Biology, Neurobiology, and Anatomy, Loyola University Medical Center, Maywood, Illinois | en_US |
dc.contributor.affiliationother | Division of Vascular Surgery, Emory University, Atlanta, Georgia | en_US |
dc.contributor.affiliationother | Department of Surgery, Loyola University Medical Center, Maywood, Illinois | en_US |
dc.contributor.affiliationother | Department of Surgery, Loyola University Medical Center, Maywood, Illinois | en_US |
dc.contributor.affiliationother | Department of Surgery, Loyola University Medical Center, Maywood, Illinois ; Department of Cell Biology, Neurobiology, and Anatomy, Loyola University Medical Center, Maywood, Illinois ; Research and Surgical Services, Edward J. Hines Jr. V.A. Hospital, Hines, Illinois ; Department of Surgery, Loyola University Medical Center, Maywood, Illinois | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/77533/1/32786_ftp.pdf | |
dc.identifier.doi | 10.1002/jbm.a.32786 | en_US |
dc.identifier.source | Journal of Biomedical Materials Research Part A | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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