Near-infrared fluorescent probe traces bisphosphonate delivery and retention in vivo
dc.contributor.author | Kozloff, Kenneth M. | en_US |
dc.contributor.author | Volakis, Leonithas I. | en_US |
dc.contributor.author | Marini, Joan C. | en_US |
dc.contributor.author | Caird, Michelle S | en_US |
dc.date.accessioned | 2010-09-02T15:21:17Z | |
dc.date.available | 2011-03-01T16:26:45Z | en_US |
dc.date.issued | 2010-08 | en_US |
dc.identifier.citation | Kozloff, Kenneth M; Volakis, Leo I; Marini, Joan C; Caird, Michelle S (2010). "Near-infrared fluorescent probe traces bisphosphonate delivery and retention in vivo." Journal of Bone and Mineral Research 25(8): 1748-1758. <http://hdl.handle.net/2027.42/77952> | en_US |
dc.identifier.issn | 0884-0431 | en_US |
dc.identifier.issn | 1523-4681 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/77952 | |
dc.description.abstract | Bisphosphonate use has expanded beyond traditional applications to include treatment of a variety of low-bone-mass conditions. Complications associated with long-term bisphosphonate treatment have been noted, generating a critical need for information describing the local bisphosphonate-cell interactions responsible for these observations. This study demonstrates that a fluorescent bisphosphonate analogue, far-red fluorescent pamidronate (FRFP), is an accurate biomarker of bisphosphonate deposition and retention in vivo and can be used to monitor site-specific local drug concentration. In vitro, FRFP is competitively inhibited from the surface of homogenized rat cortical bone by traditional bisphosphonates. In vivo, FRFP delivery to the skeleton is rapid, with fluorescence linearly correlated with bone surface area. Limb fluorescence increases linearly with injected dose of FRFP; injected FRFP does not interfere with binding of standard bisphosphonates at the doses used in this study. Long-term FRFP retention studies demonstrated that FRFP fluorescence decreases in conditions of normal bone turnover, whereas fluorescence was retained in conditions of reduced bone turnover, demonstrating preservation of local FRFP concentration. In the mandible, FRFP localized to the alveolar bone and bone surrounding the periodontal ligament and molar roots, consistent with findings of osteonecrosis of the jaw. These findings support a role for FRFP as an effective in vivo marker for bisphosphonate site-specific deposition, turnover, and long-term retention in the skeleton. © 2010 American Society for Bone and Mineral Research | en_US |
dc.format.extent | 1060887 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Anatomy and Physiology and CELL BIOLOGY | en_US |
dc.subject.other | Endocrinology | en_US |
dc.title | Near-infrared fluorescent probe traces bisphosphonate delivery and retention in vivo | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialities | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Orthopaedic Research Laboratories, Department of Orthopaedic Surgery, University of Michigan, Ann Arbor, MI, USA ; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA ; 2015 Biomedical Science Research Building, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200, USA. | en_US |
dc.contributor.affiliationum | Orthopaedic Research Laboratories, Department of Orthopaedic Surgery, University of Michigan, Ann Arbor, MI, USA ; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Orthopaedic Research Laboratories, Department of Orthopaedic Surgery, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Bone and Extracellular Matrix Branch, NICHD, NIH, Bethesda MD | en_US |
dc.identifier.pmid | 20200982 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/77952/1/66_ftp.pdf | |
dc.identifier.doi | 10.1002/jbmr.66 | en_US |
dc.identifier.source | Journal of Bone and Mineral Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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