Conformation dependence of DNA electrophoretic mobility in a converging channel
dc.contributor.author | Liao, Wei-Ching | en_US |
dc.contributor.author | Watari, Nobuhiko | en_US |
dc.contributor.author | Wang, Shengnian | en_US |
dc.contributor.author | Hu, Xin | en_US |
dc.contributor.author | Larson, Ronald G. | en_US |
dc.contributor.author | Lee, Ly James | en_US |
dc.date.accessioned | 2010-09-02T15:23:01Z | |
dc.date.available | 2011-03-01T16:26:46Z | en_US |
dc.date.issued | 2010-08 | en_US |
dc.identifier.citation | Liao, Wei-Ching; Watari, Nobuhiko; Wang, Shengnian; Hu, Xin; Larson, Ronald G.; Lee, Ly James (2010). "Conformation dependence of DNA electrophoretic mobility in a converging channel." Electrophoresis 31(16): 2813-2821. <http://hdl.handle.net/2027.42/77968> | en_US |
dc.identifier.issn | 0173-0835 | en_US |
dc.identifier.issn | 1522-2683 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/77968 | |
dc.description.abstract | The electrophoresis of Λ-DNA is observed in a microscale converging channel where the center-of-masses trajectories of DNA molecules are tracked to measure instantaneous electrophoretic (EP) mobilities of DNA molecules of various stretch lengths and conformations. Contrary to the usual assumption that DNA mobility is a constant, independent of field and DNA length in free solution, we find DNA EP mobility varies along the axis in the contracting geometry. We correlate this mobility variation with the local stretch and conformational changes of the DNA, which are induced by the electric field gradient produced by the contraction. A “shish-kebab” model of a rigid polymer segment is developed, which consists of aligned spheres acting as charge and drag centers. The EP mobility of the shish-kebab is obtained by determining the electrohydrodynamic interactions of aligned spheres driven by the electric field. Multiple shish-kebabs are then connected end-to-end to form a freely jointed chain model for a flexible DNA chain. DNA EP mobility is finally obtained as an ensemble average over the shish-kebab orientations that are biased to match the overall stretch of the DNA chain. Using physically reasonable parameters, the model agrees well with experimental results for the dependence of EP mobility on stretch and conformation. We find that the magnitude of the EP mobility increases with DNA stretch, and that this increase is more pronounced for folded conformations. | en_US |
dc.format.extent | 362888 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | WILEY-VCH Verlag | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Biochemistry and Biotechnology | en_US |
dc.title | Conformation dependence of DNA electrophoretic mobility in a converging channel | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Macromolecular Science and Engineering Center, University of Michigan, MI, USA | en_US |
dc.contributor.affiliationum | Macromolecular Science and Engineering Center, University of Michigan, MI, USA ; Department of Chemical Engineering, University of Michigan, MI, USA | en_US |
dc.contributor.affiliationother | Department of Mechanical Engineering, The Ohio State University, Columbus, OH, USA ; These authors contributed equally to this work | en_US |
dc.contributor.affiliationother | Institute for Micromanufacturing, Louisiana Tech University, LA, USA | en_US |
dc.contributor.affiliationother | Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, OH, USA | en_US |
dc.contributor.affiliationother | Department of Mechanical Engineering, The Ohio State University, Columbus, OH, USA ; Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, OH, USA ; Department of Chemical and Bimolecular Engineering, The Ohio State University, OH, USA ; Department of Chemical and Biomolecular Engineering, The Ohio State University, OH 43210, USA Fax: +1-614-292-8685 | en_US |
dc.identifier.pmid | 20737448 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/77968/1/2813_ftp.pdf | |
dc.identifier.doi | 10.1002/elps.201000081 | en_US |
dc.identifier.source | Electrophoresis | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.