Glycoprotein analysis using protein microarrays and mass spectrometry
dc.contributor.author | Patwa, Tasneem H. | en_US |
dc.contributor.author | Li, Chen | en_US |
dc.contributor.author | Simeone, Diane M. | en_US |
dc.contributor.author | Lubman, David M. | en_US |
dc.date.accessioned | 2010-09-02T15:23:08Z | |
dc.date.available | 2011-03-01T16:26:46Z | en_US |
dc.date.issued | 2010-09 | en_US |
dc.identifier.citation | Patwa, Tasneem; Li, Chen; Simeone, Diane M.; Lubman, David M. (2010). "Glycoprotein analysis using protein microarrays and mass spectrometry." Mass Spectrometry Reviews 29(5): 830-844. <http://hdl.handle.net/2027.42/77969> | en_US |
dc.identifier.issn | 0277-7037 | en_US |
dc.identifier.issn | 1098-2787 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/77969 | |
dc.description.abstract | Protein glycosylation plays an important role in a multitude of biological processes such as cell–cell recognition, growth, differentiation, and cell death. It has been shown that specific glycosylation changes are key in disease progression and can have diagnostic value for a variety of disease types such as cancer and inflammation. The complexity of carbohydrate structures and their derivatives makes their study a real challenge. Improving the isolation, separation, and characterization of carbohydrates and their glycoproteins is a subject of increasing scientific interest. With the development of new stationary phases and molecules that have affinity properties for glycoproteins, the isolation and separation of these compounds have advanced significantly. In addition to detection with mass spectrometry, the microarray platform has become an essential tool to characterize glycan structure and to study glycosylation-related biological interactions, by using probes as a means to interrogate the spotted or captured glycosylated molecules on the arrays. Furthermore, the high-throughput and reproducible nature of microarray platforms have been highlighted by its extensive applications in the field of biomarker validation, where a large number of samples must be analyzed multiple times. This review covers a brief survey of the other experimental methodologies that are currently being developed and used to study glycosylation and emphasizes methodologies that involve the use of microarray platforms. This review describes recent advances in several options of microarray platforms used in glycoprotein analysis, including glycoprotein arrays, glycan arrays, lectin arrays, and antibody/lectin arrays. The translational use of these arrays in applications related to characterization of cells and biomarker discovery is also included. © 2010 Wiley Periodicals, Inc., Mass Spec Rev 29:830–844, 2010 | en_US |
dc.format.extent | 578836 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Analytical Chemistry and Spectroscopy | en_US |
dc.title | Glycoprotein analysis using protein microarrays and mass spectrometry | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Physics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 | en_US |
dc.contributor.affiliationum | Department of Surgery and Physiology, University of Michigan Medical Center, Ann Arbor, MI | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, MI 48109 ; Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI ; Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI ; University of Michigan Medical Center, 1150 West Medical Center Drive, Building MSRB1 Rm A510B, Ann Arbor, MI 48109-0656. | en_US |
dc.contributor.affiliationother | Pfizer Global Research, Groton, CT | en_US |
dc.identifier.pmid | 20077480 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/77969/1/20269_ftp.pdf | |
dc.identifier.doi | 10.1002/mas.20269 | en_US |
dc.identifier.source | Mass Spectrometry Reviews | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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