GΑ Subunit Coordinates with Ephrin-B to Balance Self-Renewal and Differentiation in Neural Progenitor Cells
dc.contributor.author | Murai, Kiyohito | en_US |
dc.contributor.author | Qiu, Runxiang | en_US |
dc.contributor.author | Zhang, Heying | en_US |
dc.contributor.author | Wang, Jun | en_US |
dc.contributor.author | Wu, Chen | en_US |
dc.contributor.author | Neubig, Richard R. | en_US |
dc.contributor.author | Lu, Qiang | en_US |
dc.date.accessioned | 2010-10-06T14:56:39Z | |
dc.date.available | 2011-03-01T16:26:44Z | en_US |
dc.date.issued | 2010-09 | en_US |
dc.identifier.citation | Murai, Kiyohito; Qiu, Runxiang; Zhang, Heying; Wang, Jun; Wu, Chen; Neubig, Richard R.; Lu, Qiang (2010). "GΑ Subunit Coordinates with Ephrin-B to Balance Self-Renewal and Differentiation in Neural Progenitor Cells." STEM CELLS 28(9): 1581-1589. <http://hdl.handle.net/2027.42/78071> | en_US |
dc.identifier.issn | 1066-5099 | en_US |
dc.identifier.issn | 1549-4918 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/78071 | |
dc.description.abstract | Proper development of the mammalian brain requires that neural progenitor cells balance self-renewal and differentiation under precise temporal and spatial regulation, but the underlying mechanisms are not well understood. In this study, we identify GΑ subunit as a positive regulator of mammalian neurogenesis, working with the regulator of G protein signaling (RGS)-mediated ephrin-B signaling pathway as two opposing forces to maintain a balance between self-renewal and differentiation in the developing mouse cerebral cortex. Multiple GΑ i subunits are expressed by cortical neural progenitor cells during the course of cortical neurogenesis. Activation of GΑ i signaling, through in utero electroporation-mediated expression of wild-type and constitutively active GΑ i subunits, counteracts the function of ephrin-B in cortical neural progenitors to induce differentiation. Genetic knock-in of an RGS-insensitive G184SGΑ i2 causes early cell cycle exit and a reduction of cortical neural progenitor cells and leads to a defect in the production of late born cortical neurons, similar to what is observed in mutant mice with deficiency in ephrin-B reverse signaling pathway. This study reveals a role of GΑ subunit in mammalian neurogenesis and uncovers a developmental mechanism, coordinated by the GΑ and ephrin-B signaling pathways, for control of the balance between self-renewal and differentiation in neural progenitor cells. S TEM C ELLS 2010; 28:1581–1589. | en_US |
dc.format.extent | 995982 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell and Molecular Biology | en_US |
dc.title | GΑ Subunit Coordinates with Ephrin-B to Balance Self-Renewal and Differentiation in Neural Progenitor Cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationother | Department of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California, USA | en_US |
dc.contributor.affiliationother | Department of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California, USA | en_US |
dc.contributor.affiliationother | Department of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California, USA | en_US |
dc.contributor.affiliationother | Department of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California, USA ; City of Hope Graduate School of Biological Sciences, Beckman Research Institute of the City of Hope, Duarte, California, USA | en_US |
dc.contributor.affiliationother | Department of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California, USA ; City of Hope Graduate School of Biological Sciences, Beckman Research Institute of the City of Hope, Duarte, California, USA | en_US |
dc.contributor.affiliationother | Department of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California, USA ; Telephone: 626-301-8357; Fax: 626-301-8310 ; Department of Neurosicences, Beckman Research Institute of City of Hope, 1,500 East Duarte Road, Duarte, CA 91,010 | en_US |
dc.identifier.pmid | 20629171 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/78071/1/474_ftp.pdf | |
dc.identifier.doi | 10.1002/stem.474 | en_US |
dc.identifier.source | STEM CELLS | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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