Collagen I-Matrigel Scaffolds for Enhanced Schwann Cell Survival and Control of Three-Dimensional Cell Morphology
dc.contributor.author | Dewitt, Daniel D. | en_US |
dc.contributor.author | Kaszuba, Stephanie N. | en_US |
dc.contributor.author | Thompson, Deanna M. | en_US |
dc.contributor.author | Stegemann, Jan P. | en_US |
dc.date.accessioned | 2010-10-14T14:17:58Z | |
dc.date.available | 2010-10-14T14:17:58Z | |
dc.date.issued | 2009-10 | en_US |
dc.identifier.citation | Dewitt, Daniel D.; Kaszuba, Stephanie N.; Thompson, Deanna M.; Stegemann, Jan P. (2009/02/20). "Collagen I-Matrigel Scaffolds for Enhanced Schwann Cell Survival and Control of Three-Dimensional Cell Morphology." Tissue Engineering Part A, 15(10): 2785-2793 <http://hdl.handle.net/2027.42/78114> | en_US |
dc.identifier.issn | 1937-3341 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/78114 | |
dc.description.abstract | We report on the ability to control three-dimensional Schwann cell (SC) morphology using collagen I Matrigel composite scaffolds for neural engineering applications. SCs are supportive of nerve regeneration after injury, and it has recently been reported that SCs embedded in collagen I, a material frequently used in guidance channel studies, do not readily extend processes, instead adopting a spherical morphology indicative of little interaction with the matrix. We have modified collagen I matrices by adding Matrigel to make them more supportive of SCs and characterized these matrices and SC morphology in vitro. Incorporation of 10%, 20%, 35%, and 50% Matrigel by volume resulted in 2.4, 3.5, 3.7, and 4.2 times longer average SC process length after 14 days in culture than with collagen I only controls. Additionally, only 35% and 50% Matrigel constructs were able to maintain SC number over 14 days, whereas an 88% decrease in cells from initial seeding density was observed in collagen-only constructs over the same time period. Mechanical testing revealed that the addition of 50% Matrigel increased matrix stiffness from 6.4kPa in collagen I only constructs to 9.8kPa. Furthermore, second harmonic generation imaging showed that the addition of Matrigel resulted in non-uniform distribution of collagen I, and scanning electron microscope imaging illustrated distinct differences in the fibrillar structure of the different constructs. Collectively, this work lays a foundation for developing scaffolding materials that are concurrently supportive of neurons and SCs for future neural engineering applications. | en_US |
dc.format.extent | 377746 bytes | |
dc.format.extent | 3100 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Mary Ann Liebert, Inc. | en_US |
dc.title | Collagen I-Matrigel Scaffolds for Enhanced Schwann Cell Survival and Control of Three-Dimensional Cell Morphology | en_US |
dc.type | Article | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.identifier.pmid | 19231925 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/78114/1/ten.tea.2008.0406.pdf | |
dc.identifier.doi | 10.1089/ten.tea.2008.0406 | en_US |
dc.identifier.source | Tissue Engineering Part A | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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