Three-Dimensional Engineered Bone from Bone Marrow Stromal Cells and Their Autogenous Extracellular Matrix
dc.contributor.author | Syed-Picard, Fatima N. | en_US |
dc.contributor.author | Larkin, Lisa M. | en_US |
dc.contributor.author | Shaw, Charles Michael | en_US |
dc.contributor.author | Arruda, Ellen M. | en_US |
dc.date.accessioned | 2010-10-14T14:19:34Z | |
dc.date.available | 2010-10-14T14:19:34Z | |
dc.date.issued | 2009 | en_US |
dc.identifier.citation | Syed-Picard, Fatima N.; Larkin, Lisa M.; Shaw, Charles M.; Arruda, Ellen M. (2008/08/31). "Three-Dimensional Engineered Bone from Bone Marrow Stromal Cells and Their Autogenous Extracellular Matrix." Tissue Engineering Part A, 15(1): 187-195 <http://hdl.handle.net/2027.42/78137> | en_US |
dc.identifier.issn | 1937-3341 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/78137 | |
dc.description.abstract | Most bone tissue engineering research uses porous three-dimensional (3D) scaffolds for cell seeding. In this work, scaffold-less 3D bone-like tissues were engineered from rat bone marrow stromal cells (BMSCs) and their autogenous extracellular matrix (ECM). The BMSCs were cultured on a 2D substrate in medium that induced osteogenic differentiation. After reaching confluence and producing a sufficient amount of their own ECM, the cells contracted their tissue monolayer around two constraint points, forming scaffold-less cylindrical engineered bone-like constructs (EBCs). The EBCs exhibited alizarin red staining for mineralization and alkaline phosphatase activity and contained type I collagen. The EBCs developed a periosteum characterized by fibroblasts and unmineralized collagen on the periphery of the construct. Tensile tests revealed that the EBCs in culture had a tangent modulus of 7.5+/-0.5MPa at 7 days post-3D construct formation and 29+/-9MPa at 6 weeks after construct formation. Implantation of the EBCs into rats 7 days after construct formation resulted in further bone development and vascularization. Tissue explants collected at 4 weeks contained all three cell types found in native bone: osteoblasts, osteocytes, and osteoclasts. The resulting engineered tissues are the first 3D bone tissues developed without the use of exogenous scaffolding. | en_US |
dc.format.extent | 487726 bytes | |
dc.format.extent | 3100 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Mary Ann Liebert, Inc. | en_US |
dc.title | Three-Dimensional Engineered Bone from Bone Marrow Stromal Cells and Their Autogenous Extracellular Matrix | en_US |
dc.type | Article | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.identifier.pmid | 18759662 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/78137/1/ten.tea.2007.0140.pdf | |
dc.identifier.doi | 10.1089/ten.tea.2007.0140 | en_US |
dc.identifier.source | Tissue Engineering Part A | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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