Development of a serum-free system to expand dental-derived stem cells: PDLSCs and SHEDs
dc.contributor.author | Tarle, Susan A. | en_US |
dc.contributor.author | Shi, S. | en_US |
dc.contributor.author | Kaigler, Darnell | en_US |
dc.date.accessioned | 2010-11-03T15:22:09Z | |
dc.date.available | 2012-02-21T18:47:02Z | en_US |
dc.date.issued | 2011-01 | en_US |
dc.identifier.citation | Tarle, S.A.; Shi, S.; Kaigler, D. (2011). "Development of a serum-free system to expand dental-derived stem cells: PDLSCs and SHEDs." Journal of Cellular Physiology 226(1): 66-73. <http://hdl.handle.net/2027.42/78237> | en_US |
dc.identifier.issn | 0021-9541 | en_US |
dc.identifier.issn | 1097-4652 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/78237 | |
dc.description.abstract | Recently, extracted teeth have been identified as a viable source of stem cells for tissue regenerative approaches. Current expansion of these cells requires incorporation of animal sera; yet, a fundamental issue underlying cell cultivation methods for cell therapy regards concerns in using animal sera. In this study, we investigated the development of a chemically defined, serum-free media (K-M) for the expansion of human periodontal ligament stem cells (PDLSCs) and human stem cells from exfoliated deciduous teeth (SHEDs). Proliferation assays were performed comparing cells in serum-containing media (FBS-M) with cells cultured in four different serum-free medium and these demonstrated that in these medium, the cell proliferation of both cell types was significantly less than the proliferation of cells in FBS-M. Additional proliferation assays were performed using pre-coated fibronectin (FN) tissue culture plates and of the four serum-free medium, only K-M enabled PDLSCs and SHEDs to proliferate at higher rates than cells cultured in FBS-M. Next, alkaline phosphatase activity showed that PDLSCs and SHEDs exhibited similar osteogenic potential whether cultured in K-M or FBS-M, and, additionally, cells retained their multipotency in K-M as seen by expression of chondrogenic and adipogenic genes, and positive Von Kossa, Alcian blue, and Oil Red O staining. Finally, differential expression of 84 stem cell associated genes revealed that for most genes, PDLSCs and SHEDs did not differ in their expression regardless of whether cultured in K-M or FBS-M. Taken together, the data suggest that K-M can support the expansion of PDLSCs and SHEDs and maintenance of their multipotency. J. Cell. Physiol. 226: 66–73, 2010. © 2010 Wiley-Liss, Inc. | en_US |
dc.format.extent | 349726 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley Subscription Services, Inc., A Wiley Company | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Development of a serum-free system to expand dental-derived stem cells: PDLSCs and SHEDs | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Kinesiology and Sports | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Periodontics and Oral Medicine, University of Michigan, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Periodontics and Oral Medicine, University of Michigan, Ann Arbor, Michigan ; Michigan Center for Oral Health Research, University of Michigan, Ann Arbor, Michigan ; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan ; Department of Periodontics and Oral Medicine, University of Michigan, 1011 N. University, Ann Arbor, MI 48109. | en_US |
dc.contributor.affiliationother | Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles, California | en_US |
dc.identifier.pmid | 20625993 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/78237/1/22304_ftp.pdf | |
dc.identifier.doi | 10.1002/jcp.22304 | en_US |
dc.identifier.source | Journal of Cellular Physiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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