Ovalbumin sensitization and challenge increases the number of lung cells possessing a mesenchymal stromal cell phenotype

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dc.contributor.author Bentley, J KELLEY
dc.contributor.author Popova, Antonia P
dc.contributor.author Bozyk, Paul D
dc.contributor.author Linn, Marisa J
dc.contributor.author Baek, Amy E
dc.contributor.author Lei, Jing
dc.contributor.author Goldsmith, Adam M
dc.contributor.author Hershenson, Marc B
dc.date.accessioned 2010-11-04T19:16:48Z
dc.date.available 2010-11-04T19:16:48Z
dc.date.issued 2010-09-21
dc.identifier http://dx.doi.org/10.1186/1465-9921-11-127
dc.identifier.uri http://hdl.handle.net/2027.42/78265
dc.description.abstract Abstract Background Recent studies have indicated the presence of multipotent mesenchymal stromal cells (MSCs) in human lung diseases. Excess airway smooth muscle, myofibroblasts and activated fibroblasts have each been noted in asthma, suggesting that mesenchymal progenitor cells play a role in asthma pathogenesis. We therefore sought to determine whether MSCs are present in the lungs of ovalbumin (OVA)-sensitized and challenged mice, a model of allergic airways disease. Methods Balb/c mice were sensitized and challenged with PBS or OVA over a 25 day period. Flow cytometry as well as colony forming and differentiation potential were used to analyze the emergence of MSCs along with gene expression studies using immunochemical analyses, quantitative polymerase chain reaction (qPCR), and gene expression beadchips. Results A CD45-negative subset of cells expressed Stro-1, Sca-1, CD73 and CD105. Selection for these markers and negative selection against CD45 yielded a population of cells capable of adipogenic, osteogenic and chondrogenic differentiation. Lungs from OVA-treated mice demonstrated a greater average colony forming unit-fibroblast (CFU-F) than control mice. Sorted cells differed from unsorted lung adherent cells, exhibiting a pattern of gene expression nearly identical to bone marrow-derived sorted cells. Finally, cells isolated from the bronchoalveolar lavage of a human asthma patient showed identical patterns of cell surface markers and differentiation potential. Conclusions In summary, allergen sensitization and challenge is accompanied by an increase of MSCs resident in the lungs that may regulate inflammatory and fibrotic responses.
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dc.title Ovalbumin sensitization and challenge increases the number of lung cells possessing a mesenchymal stromal cell phenotype
dc.type Journal Article
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/78265/1/1465-9921-11-127.xml
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/78265/2/1465-9921-11-127.pdf
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/78265/3/1465-9921-11-127-S1.DOC
dc.language.rfc3066 en
dc.description.version Peer Reviewed
dc.rights.holder Bentley et al.; licensee BioMed Central Ltd.
dc.date.updated 2010-11-04T19:16:48Z
dc.owningcollname Interdisciplinary and Peer-Reviewed
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