Canonical Wnt/Β-catenin signaling is required for maintenance but not activation of Pitx2 expression in neural crest during eye development
dc.contributor.author | Zacharias, Amanda L. | en_US |
dc.contributor.author | Gage, Philip J. | en_US |
dc.date.accessioned | 2010-11-23T19:32:18Z | |
dc.date.available | 2011-03-01T16:26:42Z | en_US |
dc.date.issued | 2010-12 | en_US |
dc.identifier.citation | Zacharias, Amanda L.; Gage, Philip J. (2010). "Canonical Wnt/Β-catenin signaling is required for maintenance but not activation of Pitx2 expression in neural crest during eye development." Developmental Dynamics 239(12): 3215-3225. <http://hdl.handle.net/2027.42/78327> | en_US |
dc.identifier.issn | 1058-8388 | en_US |
dc.identifier.issn | 1097-0177 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/78327 | |
dc.description.abstract | Pitx2 is a paired-like homeodomain gene that acts as a key regulator of eye development. Despite its significance, upstream regulation of Pitx2 expression during eye development remains incompletely understood. We use neural crest-specific ablation of Ctnnb1 to demonstrate that canonical Wnt signaling is not required for initial activation of Pitx2 in neural crest. However, canonical Wnt signaling is subsequently required to maintain Pitx2 expression in the neural crest. Eye development in Ctnnb1 -null mice appears grossly normal early but significant phenotypes emerge following loss of Pitx2 expression. LEF-1 and Β-catenin bind Pitx2 promoter sequences in ocular neural crest, indicating a likely direct effect of canonical Wnt signaling on Pitx2 expression. Combining our data with previous reports, we propose a model wherein a sequential code of retinoic acid followed by canonical Wnt signaling are required for activation and maintenance of Pitx2 expression, respectively. Other key transcription factors in the neural crest, including Foxc1 , do not require intact canonical Wnt signaling. Developmental Dynamics 239:3215–3225, 2010. © 2010 Wiley-Liss, Inc. | en_US |
dc.format.extent | 3867267 bytes | |
dc.format.extent | 3118 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.publisher | Wiley-Liss, Inc. | en_US |
dc.subject.other | Life and Medical Sciences | en_US |
dc.subject.other | Cell & Developmental Biology | en_US |
dc.title | Canonical Wnt/Β-catenin signaling is required for maintenance but not activation of Pitx2 expression in neural crest during eye development | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Pediatrics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Ophthalmology and Visual Sciences, and Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Departments of Ophthalmology and Visual Sciences, and Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan ; Department of Ophthalmology & Visual Sciences, University of Michigan Medical School, 305 Kellogg Eye Center, 1000 Wall Street, Ann Arbor, MI 48105 | en_US |
dc.identifier.pmid | 20960542 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/78327/1/22459_ftp.pdf | |
dc.identifier.doi | 10.1002/dvdy.22459 | en_US |
dc.identifier.source | Developmental Dynamics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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