Now showing items 11-20 of 70
Purification, properties, and oxygen reactivity of p-hydroxybenzoate hydroxylase from Pseudomonas aeruginosa
(Elsevier, 1989-12-21)
The monooxygenase, p-hydroxybenzoate hydroxylase (4-hydroxybenzoate, NADPH: oxygen oxidoreductase (3-hydroxylating), EC 1.14.13.2) has been isolated and purified from Pseudomonas aeruginosa. The reaction catalysed is linked ...
Chemical characterization of biodegradative threonine dehydratases from two enteric bacteria
(Elsevier, 1982-08-23)
Some chemical properties of the purified biodegredative threonine dehydratases (-threonine hydro-lyase (deaminating), EC 4.2.1.16) from Escherichia coli and Salmonella typhimurium are described. The overall amino acid ...
Comparison of the phospholipid requirements and molecular form of CTP: Phosphocholine cytidylyltransferase from rat lung, kidney, brain and liver
(Elsevier, 1980-12-05)
The Cytidylyltransferase activity in fresh cytosol from different tissues of the rat was measured in the absence and presence of phosphatidylglycerol. In all cases addition of this lipid produced large increases in enzyme ...
Binding of hydrophobic ligands to plant lectins: Titration with arylaminonaphthalenesulfonates
(Elsevier, 1983-07-15)
Binding of the Hydrophobic ligands 1,8-anilinonaphthalenesulfonic acid (ANS) and 2,6-toluidinylnaphthalenesulfonic acid (TNS) to a variety of plant lectins was studied by lectin-induced alteration of the fluorescence spectra ...
Isolation and characterization of a glycopeptide from human senescent erythrocytes
(Elsevier, 1983-08-16)
A glycopeptide (called "senescence-factor glycopeptide", SF-G) has been isolated from a tryptic digest of human erythrocytes by specific adsorption and elution from immobilized peanut lectin. SF-G was detectable in old but ...
Muscarinic receptor-stimulated Ca2+ signaling and inositol lipid metabolism in avian salt gland cells
(Elsevier, 1986-11-28)
Activation of muscarinic cholinergic receptors was studied by measuring agonist-stimulated inositol lipid turnover and changes in [Ca2+]i in dissociated salt gland secretory cells. Carbachol stimulation of quin2-loaded ...
The activity and properties of an acidic triacylglycerol lipase from adult and fetal rat lung
(Elsevier, 1986-01-03)
Triacylglycerol lipase with maximal activity at pH 5 was present in adult and fetal lung. The activity was inhibited by serum concentrations used to measure lipoprotein lipase and by 0.5 M NaCl. The activity in homogenates ...
Role of alcohol P-450-oxygenase (APO) in microsomal ethanol oxidation
(Elsevier, 1985)
The form of liver microsomal cytochrome P-450 induced by chronic administration of ethanol to rabbits, designated as P-450 or P-450 isozyme 3a, has been purified to homogeneity as judged by several criteria, including NH2- ...
One-step purification of murine IgM and human [alpha]2-macroglobulin by affinity chromatography on immobilized snowdrop bulb lectin
(Elsevier, 1988-12)
A new mannose-specific plant lectin (GNA) isolated from the snowdrop bulb was immobilized on Sepharose 4B and employed for the purification of certain glycoproteins with high-mannose type glycan chains. Murine IgM bound ...
Production and characterization of antisera raised to the manninotrionate group [[alpha]--Galp-(1-->6)-[alpha]--Galp-(1-->6)--gluconate]
(Elsevier, 1989-06-15)
Manninotrionate [[alpha]--Galp-(1-->6)-[alpha]--Galp-(1-->6)--gluconate] was coupled to bovine serum albumin (BSA), and antisera were raised in rabbits to this carbohydrate--protein conjugate. The antisera were studied ...