Now showing items 61-70 of 70
Reexamination of the carbohydrate binding stoichiometry of lima bean lectin
(Elsevier, 1984-04)
The carbohydrate binding stoichiometry of lima bean lectin component III was reexamined using equilibrium dialysis and quantitative affinity chromatography following limited chemical modification. Equilibrium dialysis ...
Preparative isolation of peroxisomes from liver and kidney using Metrizamide density gradient centrifugation in a vertical rotor
(Elsevier, 1985-07)
A method for the preparative isolation of peroxisomes from the livers of rat, guinea pig, and mouse, and also from rat kidney is described. The light mitochondrial fraction, i.e., particles sedimenting between 33,000 and ...
Glycosyltransferase activities of Ehrlich ascites tumor cells: Detection, isolation, and characterization using oligosaccharide-Synsorb beads
(Elsevier, 1987-04)
Detergent extracts of Ehrlich tumor cell membranes exhibit a host of glycosyltransferase activities which have been investigated using oligosaccharides immobilized to Synsorb beads as acceptors. Glycosidase digestions in ...
Dimerization occurs during the reversible acid inactivation of 2-oxo-4-hydroxyglutarate aldolase from Escherichia coli
(Elsevier, 1983-11-28)
Exposure of Escherichia coli 2-oxo-4-hydroxyglutarate aldolase (4-hydroxy-2-oxoglutarate glyoxylate-lyase, EC 4.1.3.16) (molecular WEIGHT = 63000) to phosphoric acid at pH 1.6 for 10 min at 4[delta]C causes 95% or greater ...
Synthesis of [alpha]- and [beta]-glycosides containing spin labels, as probes for studies of carbohydrate-protein interaction
(Elsevier, 1981-03-02)
Nitroxide spin-labeled [alpha]--glycopyranosides were synthesized in good yield and in a highly stereoselective manner by reaction of per-O-benzyl-[alpha]--glycopyranosyl bromides with 2,2,6,6-tetramethyl-4-piperidinol ...
Determination of the dead time of a stopped-flow fluorometer
(Elsevier, 1989-09)
This investigation was carried out to develop a convenient alternative method for examining the performance and determining the dead time of a stopped-flow fluorometer. We examined the kinetics for the formation of the ...
Structure of the dextran of Leuconostoc mesenteroides B-1355
(Elsevier, 1980-09-15)
The structure of dextran B-1355-S (soluble fraction) has been investigated. Acid hydrolysis of the methylated dextran yielded 2,3,4,6-tetra-, 2,3,4-tri-, 2,4,6-tri-, and 2,4-di-O-methyl--glucose, in the molar ratios of ...
Characterization of cytosolic forms of CTP: choline-phosphate cytidylyltransferase in lung, isolated alveolar type II cells, A549 cell and Hep G2 cells
(Elsevier, 1989-12-18)
The subcellular forms of cytidylyltransferase (EC 2.7.7.15) in rat lung, rat liver, Hep G2 cells, A549 cells and alveolar Type II cells from adult rats were separated by glycerol density centrifugation. Cytosol prepared ...
Assay and properties of the enzyme catalyzing the biosynthesis of 1-O-alkyl dihydroxyacetone 3-phosphate
(Elsevier, 1981-10-01)
The properties of the enzyme alkyl dihydroxyacetone phosphate synthase (1-O-acyl dihydroxyacetone 3-phosphate + ROH --> 1-O-alkyl dihydroxyacetone 3-phosphate + fatty acid) in Ehrlich ascites tumor cell microsomes were ...
[omega]-1 and [omega]-2 hydroxylation of prostaglandins by rabbit hepatic microsomal cytochrome P-450 isozyme 6
(Elsevier, 1985-11-15)
Incubation of prostaglandin E1 (PGE1) with liver microsomes from control rabbits and from rabbits treated with ethanol or imidazole yielded 18-, 19-, and 20-hydroxy metabolites, representing hydroxylation at [omega]-2, ...