Now showing items 21-30 of 48
Folylpolyglutamates as substrates and inhibitors of folate-dependent enzymes
(Elsevier, 1987)
The true intracellular substrates for folate-dependent enzymes are folylpolyglutamates. We have used measurements of the Ki values of folylpolyglutamate dead end inhibitors to assess the relative affinities of folate-dependent ...
Bovine prothrombin does not contain [alpha]--galactopyranosyl groups
(Elsevier, 1980-02)
Using purified lectins (Bandeiraea simplicifolia I-B4 isolectin and the castor bean agglutinin) and glycosidases ([alpha]- and [beta]-galactosidases) we have shown that the terminal, non-reducing galactopyranosyl groups ...
Solubilization and partial purification of dihydroxyacetone-phosphate acyltransferase from guinea pig liver
(Elsevier, 1983-10-01)
Dihydroxyacetone-phosphate:acyl coenzyme A acyltransferase (EC 2.3.1.42) was solubilized and partially purified from guinea pig liver crude peroxisomal fraction. The peroxisomal membrane was isolated after osmotic shock ...
Immunochemical evidence for a role of cytochrome P-450 in liver microsomal ethanol oxidation
(Elsevier, 1984-11-15)
Antibodies to cytochrome P-450 isozyme 3a, the ethanol-inducible isozyme in rabbit liver, were used to determine the role of this enzyme in the microsomal oxidation of alcohols and the p-hydroxylation of aniline. P-450 ...
Enzyme-linked lectin assay (ELLA): Use of alkaline phosphatase-conjugated Griffonia simplicifolia B4 isolectin for the detection of [alpha]--galactopyranosyl end groups
(Elsevier, 1983-04-15)
Alkaline phosphatase has been coupled to Griffonia simplicifolia I B4 isolectin using a one-step glutaraldehyde conjugation procedure. This enzyme-lectin conjugate (AP-GS I-B4) has been used to specifically detect plastic-bound ...
Modulation of methylenetetrahydrofolate reductase activity by S-adenosylmethionine and by dihydrofolate and its polyglutamate analogues
(Elsevier, 1982)
Methylenetetrahydrofolate reductase catalyzes the reduction of methylenetetrahydrofolate ot methyltetrahydrofolate. This reaction commits one carbon units to the pathways of adenosylmethionine-dependent methylation in ...
Carbohydrate binding studies on the lectin from Datura stramonium seeds
(Elsevier, 1984-06)
The carbohydrate-binding properties of the Datura stramonium seed lectin were studied by equilibrium dialysis, quantitative precipitation of natural and synthetic glycoproteins, and hapten inhibition of precipitation. The ...
Characterization of the extended carbohydrate binding site of concanavalin A: Specificity for interaction with the nonreducing termini of [alpha]-(1-->2)-linked disaccharides
(Elsevier, 1981-07)
p-Nitrophenyl 2-O-[alpha]--galactopyranosyl-[alpha]--mannopyranoside and p-nitrophenyl 2-O-[alpha]--glucopyranosyl-[alpha]--mannopyranoside were synthesized and the interactions of these disaccharides with concanavalin A ...
A rapid method for the isolation of peroxisomes from rat liver
(Elsevier, 1986-11-15)
A preparative method for the isolation of peroxisomes from the liver of normal, untreated rats is described. The peroxisome-enriched "light mitochondrial" fraction is layered on a 30% Nycodenz (5-[N-2,3-dihydroxypropylac ...
Lectin-binding domains on laminin
(Elsevier, 1983-11)
The nature and location of carbohydrate moieties on the laminin molecule were identified by studying the binding affinity of a series of lectins for purified, proteasederived fragments of laminin. Laminin is a cross-shaped ...