Now showing items 21-30 of 57
New mannose-specific lectins from garlic (Allium sativum) and ramsons (Allium ursinum) bulbs
(Elsevier, 1992-05-22)
Two new mannose-binding lectins were isolated from garlic (Allium sativum, ASA) and ramsons (Allium ursinum, AUA) bulbs, of the family Alliaceae, by affinity chromatography on immobilized mannose. The carbohydrate-binding ...
Substitution of D-Trp 32 in NPY Destabilizes the Binding Transition State to the Y1 Receptor Site in SK-N-MC Cell Membranes
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 1997-04)
The retention rate of the spin label 3-isothiocyanto methyl-2,2,5,5-tetramethyl-1-pyrrolidinyl oxyl spin label (proxyl) attached to the porcine N-acetyl-NPY peptide and the porcine N-acetyl-D-Trp 32 -NPY peptide at Lys 4 ...
HCCH-TOCSY spectroscopy of 13 C-labeled proteins in H 2 O using heteronuclear cross-polarization and pulsed-field gradients
(Kluwer Academic Publishers; ESCOM Science Publishers B.V. ; Springer Science+Business Media, 1995-02)
A pulsed-field gradient-enhanced, heteronuclear cross-polarization-driven, 3D HCCH-TOCSY experiment is described, which in a single scan can achieve nearly ideal solvent suppression for protein samples in H 2 O solution. ...
PKC Activity and PKC-[alpha] mRNA Content Are Reduced in Serum-Deprived Human Neuroblastoma Cells without Concomitant Induction of Differentiation
(Elsevier, 1993-08)
Protein kinase C (PKC) is a serine/threonine kinase which is thought to play an important role in cellular proliferation and differentiation. PKC activity is stimulated physiologically by diacylglycerol and experimentally ...
Cobalamin-dependent methionine synthase: the structure of a methylcobalamin-binding fragment and implications for other B12-dependent enzymes
(Elsevier, 1994)
Cobalamin-dependent methionine synthase is a large enzyme composed of structurally and functionally distinct regions. Recent studies have begun to define the roles of several regions of the protein. In particular, the ...
Study of protein dynamics in solution by measurement of 13 C α - 13 CO NOE and 13 CO longitudinal relaxation
(Kluwer Academic Publishers; ESCOM Science Publishers B.V. ; Springer Science+Business Media, 1996-03)
13 C α - 13 CO homonuclear NOE and 13 CO T 1 relaxation were measured for a 20 kDa protein using tripleresonance pulse sequences. The experiments were sufficiently sensitive to obtain statistically significant differences ...
Cloning and characterization of the lectin cDNA clones from onion, shallot and leek
(Kluwer Academic Publishers; Springer Science+Business Media, 1993-10)
Characterization of the lectins from onion ( Allium cepa ), shallot ( A. ascalonicum ) and leek ( A. porrum ) has shown that these lectins differ from previously isolated Alliaceae lectins not only in their molecular ...
Expression of rabbit cytochrome P-450IIE2 in yeast and stabilization of the enzyme by 4-methylpyrazole
(Elsevier, 1990-11-15)
A rabbit cytochrome P-450IIE2 full-length cDNA was cloned into a yeast episomal plasmid (YEp13) between the copper-responsive yeast metallothionein gene promoter (CUP1) and the iso-1-cytochrome c gene terminator (CYC1), ...
Spatial and temporal immunocytochemical analysis of drosulfakinin (Dsk) gene products in the Drosophila melanogaster central nervous system
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 1995-12)
The spatial and temporal distribution of three peptides, DSK I, DSK II, and DSK 0, encoded by the Drosophila melanogaster drosulfakinin (Dsk) gene, have been examined in the central nervous system. DSK I and DSK II have a ...
Expression of Corticotropin-Releasing Hormone Transgenes in Neurons of Adult and Developing Mice
(Elsevier, 1994-12)
The DNA sequences important for cell-specific expression and developmental regulation of corticotropin-releasing hormone (CRH) were analyzed in transgenic mice. A construct containing 0.5 kb of CRH 5' flanking DNA linked ...