Now showing items 11-20 of 22
HCCH-TOCSY spectroscopy of 13 C-labeled proteins in H 2 O using heteronuclear cross-polarization and pulsed-field gradients
(Kluwer Academic Publishers; ESCOM Science Publishers B.V. ; Springer Science+Business Media, 1995-02)
A pulsed-field gradient-enhanced, heteronuclear cross-polarization-driven, 3D HCCH-TOCSY experiment is described, which in a single scan can achieve nearly ideal solvent suppression for protein samples in H 2 O solution. ...
Study of protein dynamics in solution by measurement of 13 C α - 13 CO NOE and 13 CO longitudinal relaxation
(Kluwer Academic Publishers; ESCOM Science Publishers B.V. ; Springer Science+Business Media, 1996-03)
13 C α - 13 CO homonuclear NOE and 13 CO T 1 relaxation were measured for a 20 kDa protein using tripleresonance pulse sequences. The experiments were sufficiently sensitive to obtain statistically significant differences ...
Cloning and characterization of the lectin cDNA clones from onion, shallot and leek
(Kluwer Academic Publishers; Springer Science+Business Media, 1993-10)
Characterization of the lectins from onion ( Allium cepa ), shallot ( A. ascalonicum ) and leek ( A. porrum ) has shown that these lectins differ from previously isolated Alliaceae lectins not only in their molecular ...
Expression of rabbit cytochrome P-450IIE2 in yeast and stabilization of the enzyme by 4-methylpyrazole
(Elsevier, 1990-11-15)
A rabbit cytochrome P-450IIE2 full-length cDNA was cloned into a yeast episomal plasmid (YEp13) between the copper-responsive yeast metallothionein gene promoter (CUP1) and the iso-1-cytochrome c gene terminator (CYC1), ...
Spatial and temporal immunocytochemical analysis of drosulfakinin (Dsk) gene products in the Drosophila melanogaster central nervous system
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 1995-12)
The spatial and temporal distribution of three peptides, DSK I, DSK II, and DSK 0, encoded by the Drosophila melanogaster drosulfakinin (Dsk) gene, have been examined in the central nervous system. DSK I and DSK II have a ...
Sensitivity improvement in 2D and 3D HCCH spectroscopy using heteronuclear cross-polarization
(Kluwer Academic Publishers; ESCOM Science Publishers B.V. ; Springer Science+Business Media, 1993-07)
A new method, which employs a sequence of heteronuclear-homonuclear-heteronuclear Hartmann-Hahn (HEHOHEHAHA) cross-polarization steps for obtaining through-bond H-C-C-H correlations in larger proteins (M r > 15 kDa), is ...
Line narrowing in spectra of proteins dissolved in a dilute liquid crystalline phase by band-selective adiabatic decoupling: Application to 1HN-15N residual dipolar coupling measurements
(Kluwer Academic Publishers; Springer Science+Business Media, 1999-12)
Residual heteronuclear dipolar couplings obtained from partially oriented protein samples can provide unique NMR constraints for protein structure determination. However, partial orientation of protein samples also causes ...
Lack of correspondence between the room-temperature phosphorescence decay-components and Trp residues in a series of Trp-->Cys or Trp-->Phemutants of human carbonic anhydrase II
(Elsevier, 1994-10-17)
The room temperature phosphorescence of native human carbonic anhydrase (CA), and several mutants of this enzyme has been investigated. In these mutants the seven tryptophan residues in the native protein have sequentially ...
Crystallization and preliminary X-ray diffraction studies of the cobalamin-binding domain of methionine synthase from Escherichia coli
(Elsevier, 1992-05-20)
Crystals of a cobalamin-binding domain (Mr = 28,000) have been grown in polyethylene glycol 6000 at pH 7.5, starting from solutions of intact (Mr = 133,000) cobalamin-dependent methionine synthase. The crystals are ...
Localization of the corticotropin-releasing hormone receptor gene on mouse Chromosome 11
(Springer-Verlag, 1995-01)