Now showing items 1-10 of 25
Detection of intermediate protein conformations by room temperature tryptophan phosphorescence spectroscopy during denaturation of Escherichia coli alkaline phosphatase
(Elsevier, 1993-12)
The reversible denaturation of Escherichia coli alkaline phosphatase (AP) was followed by monitoring changes in enzymatic activity as well as by measurements of the time-resolved room temperature phosphorescence from Trp ...
Coupling of dihydroriboflavin oxidation to the formation of the higher valence states of hemeproteins
(Elsevier, 1991-11-27)
SummaryThe reactions between hydrogen peroxide and hemeproteins have been coupled to the oxidation of dihydroriboflavin so as to provide a simple method for measuring the rate constant of hemeprotein peroxidation. ...
Structure-function analysis in nuclear RNase P RNA
(Kluwer Academic Publishers; Springer Science+Business Media, 1995-06)
Eukaryotic ribonuclease P (RNase P) enzymes require both RNA and protein subunits for activity in vivo and in vitro . We have undertaken an analysis of the complex RNA subunit of the nuclear holoenzyme in an effort to ...
Identification and localization of an immunoreactiveAMPA-type glutamate receptor subunit (GluR4) with respect to identified photoreceptor synapses in the outer plexiform layer of goldfish retina
(Kluwer Academic Publishers; Chapman and Hall ; Springer Science+Business Media, 1997-10)
L-glutamate, the main excitatory synaptic transmitter in the retina, is released from photoreceptors and evokes responses in second-order retinal neurons (horizontal, bipolar cells) which utilize both ionotropic and ...
High-Resolution Detection of Five Frequencies in a Single 3D Spectrum: HNHCACO - a Bidirectional Coherence Transfer Experiment
(Kluwer Academic Publishers; Springer Science+Business Media, 1998-02)
A new triple-resonance pulse sequence, 3D HNHCACO, is introduced and discussed, which identifies sequential correlations of the backbone nuclei (Hα(i-1), Cα(i-1), C′(i-1), NH(i)) of doubly labeled proteins in H2O. The ...
pH-Dependent Decarboxylation of 2-Amino-3-ketobutyrate, the Unstable Intermediate in the Threonine Dehydrogenase-Initiated Pathway for Threonine Utilization
(Elsevier, 1993-02-15)
2-Amino-3-ketobutyrate can be readily formed enzymatically by the action of L-threonine dehydrogenase. A convenient assay for determining the half-life of this [beta]-keto acid is afforded by its rapid and quantitative ...
Corticotropin-releasing hormone ( Crh ) maps to mouse Chromosome 3
(Springer-Verlag; Springer-Verlag New York, Inc., 1993-10)
Rabbit nasal cytochrome P-450 NMa has high activity as a nicotine oxidase
(Elsevier, 1990-01-30)
Rabbit nasal olfactory and respiratory microsomes demonstrate high activity toward [3H]-(S)-nicotine, with specific activities of 22.2 and 6.5 nmol/min/mg protein, respectively. The major metabolite produced is (S)-nicotine ...
Binding of yeast TFIIIC to tRNA gene bipartite internal promoters: Analysis of physical effects on the intervening DNA
(Elsevier, 1991-04-30)
Complexes between transcription factor TFIIIC and eukaryotic tRNA gene internal promoter A and B boxes are unusual in that the binding to the two distinct sites tolerates considerable variation in both distance and helical ...
Band-selective hetero- and homonuclear cross-polarization using trains of shaped pulses
(ESCOM Science Publishers B.V., Leiden/Kluwer Academic Publishers; ESCOM Science Publishers B.V. ; Springer Science+Business Media, 1996-09)
The performance of solution cross-polarization using trains of shaped pulses on two channels is investigated by computer simulation and experiment. It is determined that a Waltz modulation pattern of Gaussian pulses of ...