Now showing items 1-10 of 11
Renewed interest in the polyphosphoinositides
(Elsevier, 1984-02)
The significance of the enhanced cellular phosphatidate and phosphatidylinositol turnover which occurs in response to specific extracellular messengers has been the subject of much interest and speculation. Until recently, ...
Acetylcholine stimulates hydrolysis of 32P-labeled phosphatidic acid in guinea pig synaptosomes
(Elsevier, 1973-02-05)
[3H]-inositol or [3H]-arachidonate was injected intracerebrally into guinea pigs. Labeled nerve endings were incubated with Ach* or CCh, both of which stimulate labeling of PhA and PhI from 32Pi by > 100% and 70% ...
II. Biochemical mechanisms in the phosphatidylinositol effect
(Elsevier, 1983-05-02)
Stimulated labeling of phospholipids from 32Pi is a hallmark of activation of a variety of cell surface receptors. In the case of the central nervous system, the response can reflect muscarinic activation. Recent studies ...
Rapid expression of novel proteins in goldfish retina following optic nerve crush
(Elsevier, 1986-08-14)
SummaryPolyadenylated messenger RNA was isolated from goldfish retinas at various times following unilateral crush of the optic nerve. RNA was translated in a cell-free system and product proteins analyzed by two-dimensional ...
Muscarinic stimulation of phospholipid turnover in dissociated avian salt gland cells
(Elsevier, 1983-05-02)
Addition of carbamylcholine to 32P-prelabeled dissociated avian salt gland cells resulted in increased turnover of phosphatidic acid, phosphatidylinositol, phosphatidylinositol 4-phosphate and phosphatidylinositol ...
Tetraphenylborate stimulation of phospholipid labeling in goldfish brain
(Elsevier, 1969-05-08)
The presence of tetraphenylborate (TPB, 10-3 M to 10-4 M) in goldfish brain particulate preparations during a 5-15 min incubation with [gamma]-32P-ATP or [gamma]-33P-ATP results in marked stimulation of labeling of ...
Formation of 3'-32P-acyl CoA from [gamma]-32P-ATP in guinea pig liver mitochondrial extracts
(Elsevier, 1972-01-31)
SummaryA soluble extract that catalyzes the exchange of acyl groups from acyl coenzyme A to coenzyme A has been obtained from guinea pig liver mitochondria. None of several known acyl group acceptors stimulates the transfer ...
Phosphodiesteratic breakdown of endogenous polyphosphoinositides in nerve ending membranes
(Elsevier, 1983-05-16)
When a membrane preparation, obtained by freezing and thawing nerve endings labeled by preincubation with 32Pi, is incubated in the presence of millimolar Ca2+, there is a rapid and selective loss of label from the ...
Release of RNA from goldfish brain nuclei by sodium dodecyl sulfate
(Elsevier, 1968-02-15)
Labeling of mitochondrial phosphatidyl inositol phosphate by Pi32 and by [gamma]P32ATP
(Elsevier, 1965-07-12)
Garbus et al., 1963 reported labeling of mitochondrial phospholipid following short incubations with Pi32. The rapidly labeled lipid has been identified by means of alkaline hydrolysis as phosphatidyl inositol phosphate ...