Now showing items 1-10 of 31
Micro method for determination of borohydride with NAD+
(Elsevier, 1973-08)
A spectrophotometric method for the determination of borohydride is described. It involves the reduction of NAD+ to a number of isomeric forms of NADH by borohydride. In the standard assay procedure herewith presented, ...
A game to help students comprehend enzyme structure and mechanism of action
(Elsevier, 1976-01)
The inhibitive effects of steroid analogues in the binding of tritiated 5[alpha]-dihydrotecto3terone to receptor proteins from rat prostate tissue
(Elsevier, 1975-02)
The relationship between molecular structure and the binding potential of steroids to receptor proteins was investigated. Twenty-four selected steroids were studied in minced incubations of rat pro state tissue. Measurements ...
Quantitative determination of reducing sugars, oligosaccharides, and glycoproteins with [3H]borohydride
(Elsevier, 1973-09)
A very simple and direct radioactive isotope method has been described for the quantitative determination of reducing sugars. The method can be adapted to give any degree of sensitivity desired. The procedure is of general ...
The purification and properties of an aryl [beta]-hexosidase from bovine liver
(Elsevier, 1977-01-30)
An aryl [beta]-hexosidase was purified 800-fold from bovine liver. The purified enzyme hydrolyzed p-nitrophenyl glycosylpyranoside derivatives of [beta]--galactose, [beta]--glucose, [beta]--xylose, [beta]--mannose, and ...
Biosynthesis of keratan sulfate: Purification and properties of a galactosyltransferase from bovine cornea
(Elsevier, 1979-02)
A soluble galactosyltransferase was purified 22,000-fold from bovine cornea. The enzyme catalyzes the transfer of galactose from UDP-galactose to N-acetyl--glucosamine, [alpha]- and [beta]-glucosaminides, bovine cornea and ...
Studies on hydroperoxide-dependent substrate hydroxylation by purified liver microsomal cytochrome P-450
(Elsevier, 1976-08)
Highly purified liver microsomal cytochrome P-450 catalyzes the hydroperoxide-dependent hydroxylation of a variety of substrates in the absence of NADPH, NADPH-cytochrome P-450 reductase, and molecular oxygen. The addition ...
Characterization of the [omega]-hydroxylase of Pseudomonas oleovorans as a nonheme iron protein
(Elsevier, 1977-10)
The [omega]-hydroxylase of Pseudomonas oleovorans, which catalyzes the hydroxylation of fatty acids and alkanes and the epoxidation of alkenes in the presence of a reduced pyridine nucleotide, a reductase, rubredoxin, and ...