Now showing items 1-10 of 16
GTP[gamma]S-stimulated hydrolysis of phosphatidyinositol-4,5-bisphosphate by soluble phospholipase C from human platelets requires soluble GTP-binding protein
(Elsevier, 1988-07-15)
GTP-binding activity was fractionated into two peaks (GI and GII) by chromatography on heparin-agarose. GTP-dependent PLC activity eluted as a single peak, which co-chromatographed with GTP-binding peak GII. Rechromatography ...
Differential activation of human skin cells by platelet activating factor: Stimulation of phosphoinositide turnover and arachidonic acid mobilization in keratinocytes but not in fibroblasts
(Elsevier, 1989-09-29)
Treatment of cultured adult human keratinocytes with platelet activating factor (PAF) resulted in a rapid, dose dependent accumulation of inositol phosphates. Inositol trisphosphate (IP3), inositol bisphosphate (IP2) and ...
Retinoic acid and synthetic analogs differentially activate retinoic acid receptor dependent transcription
(Elsevier, 1990-11-30)
SummaryWe have developed an assay where the potency of retinoids in retinoic acid receptor (RAR) mediated transcriptional activation can be rapidly evaluated. In this assay hRAR-[alpha], hRAR-[beta] and hRAR-[gamma] were ...
Ultraviolet irradiation-induces epidermal growth factor receptor (EGFR) nuclear translocation in human keratinocytes
(Wiley Subscription Services, Inc., A Wiley Company, 2009-08-01)
Epidermal growth factor receptor (EGFR) plays a critical role in mediating ultraviolet (UV) irradiation-induced signal transduction and gene expression in human keratinocytes. EGFR activation results from increased ...
Regulation of TGF-Α expression in human keratinocytes: PKC-dependent and -independent pathways
(Wiley Subscription Services, Inc., A Wiley Company, 1992-05)
Transforming growth factor–Α (TGF-Α) is an autocrine growth factor for epidermal keratinocytes that can induce its own expression (autoinduction). Because the regulation of this process may be important for the control of ...
Mitochondrial protein p26 BCL2 reduces growth factor requirements of NIH3T3 fibroblasts
(Elsevier, 1991-08)
The BCL2 (B cell lymphoma/leukemia-2) proto-oncogene encodes a 26-kDa protein that has been localized to the inner mitochondrial membrane and that has been shown to enhance the survival of some types of hematopoietic cells. ...
A retinoic acid-inducible skin-specific gene (RIS-1/psoriasin): molecular cloning and analysis of gene expression in human skin in vivo and cultured skin cells in vitro
(Kluwer Academic Publishers; Springer Science+Business Media, 1994-06)
A retinoic acid (RA) inducible skin-specific gene transcript (RIS-1) was isolated by differential hybridization screening of a RA-treated human skin cDNA library. The library was constructed from pooled RNA derived from ...
ELAM-1-dependent cell adhesion to vascular endothelium determined by a transfected human fucosyltransferase cDNA
(Elsevier, 1990-11-02)
Adhesion of circulating leukocytes to the vascular endothelium during inflammation is mediated in part by their interaction with the endothellal-leukocyte adhesion molecule ELAM-1. ELAM-1, a member of the LEC-CAM family ...
Phosphatidic acid and phospholipase D both stimulate phosphoinositide turnover in cultured human keratinocytes
(Elsevier, 1993-11)
Phosphatidic acid (PA) induced a rapid dose-dependent increase in production of inositol phosphates in cultured adult human keratinocytes, peaking at 30 s. Natural and dioleoyl PA were equally effective, while other ...
GTP and cytosol stimulate phosphoinositide hydrolysis in isolated platelet membranes
(Elsevier, 1986-06-13)
Hydrolysis of polyphosphoinositides by phospholipase C was examined in isolated membranes prepared from [32P]labelled platelets. In the presence of GTP[gamma]S, thrombin increased the release of inositol triphosphate and ...