Now showing items 1-10 of 12
Effects of serum and insulin-like growth factors on human neuroblastoma cell growth
(Elsevier, 1993-10-20)
Insulin-like growth factors (IGF-I and IGF-II) are mitogenic polypeptides expressed in both developing and adult tissues. To examine the effects of IGFs on neuronal growth, we used SH-SY5Y neuroblastoma cells as an in vitro ...
A two-step procedure for purification of papain from extract of papaya latex
(Elsevier, 1974-09)
A method is presented for purifying papain from extracts of papaya latex. The procedure involves precipitation of the extract of papaya latex with sodium chloride followed by affinity chromatography of the redissolved ...
Characterization of a mutant human erythrocyte carbonic anhydrase: Carbonic anhydrase IcGuam: The amino acid substitution and carboxylesterase and hydratase activities
(Elsevier, 1966-11)
Evidence is presented which indicates that an arginine residue has substituted for a glycine residue in the abnormal human erythrocyte carbonic anhydrase, CA IcGuam. The ratio of the amount of mutant to normal enzyme ...
Genetic variation in the carbonic anhydrase isozymes of macaque monkeys : III. Biosynthesis of carbonic anhydrases in bone marrow erythroid cells and peripheral blood reticulocytes of Macaco nemestrina
(Elsevier, 1973-09)
The carbonic anhydrase isozymes (CA I and CA II) of the pig-tailed macaque, Macaca nemestrina, have been chosen to study the regulation of enzyme levels in red blood cells. Two quantitative variants of CA I that are ideal ...
Reactivation and immunological studies on a japanese variant of red cell carbonic anhydrase I, CA I hiroshima-1
(Elsevier, 1982)
1. 1. Under certain conditions, CA I HIR-1++ (86 Asp--> Gly) was inactivated in Gnd-HCl more rapidly than normal CA I.2. 2. After denaturation in 5.0 M Gnd [middle dot] HC1, CA I HIR-1 was reactivated more slowly than ...
IGF receptor function and regulation in autocrine human neuroblastoma cell growth
(Elsevier, 1993-10-20)
Insulin-like growth factor-II (IGF-II) and its receptors (type I and II IGF receptors) are expressed in the nervous system in a tissue and developmentally specific manner. We have previously shown that SH-SY5Y human ...
Paradoxical effects of glucocorticoids on regulation of plasminogen activator activity : Mediation by glucocorticoid receptors
(Elsevier, 1984-02)
Dexamethasone, a synthetic glucocorticoid, decreases the plasminogen activator (PA) activity of HTC rat hepatoma cells in tissue culture. Paradoxically, dexamethasone enhances the cyclic nucleotide stimulation of PA activity ...
Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli
(Elsevier, 1990-12)
DNA polymerase from Thermus aquaticus has become a common reagent in molecular biology because of its utility in DNA amplification and DNA sequencing protocols. A simplified method is described here for isolating the ...
Dissociation constants for carbonic anhydrase-sulfonamide binding by high-performance liquid chromatography
(Elsevier, 1984-03)
Carbonic anhydrase-azosulfonamide dissociation constants (Kd) were determined by gel filtration with high-performance liquid chromatography. By measuring the area of the elution peak at two wavelengths. Kd values were ...