Now showing items 31-40 of 83
Development of the Nude Rabbit Model
(Elsevier, 2021-03-09)
CRISPR-Cas9-mediated gene knockout of FOXN1 in rabbits leads to the establishment of the nude rabbit (NuRabbit) colony, characterized by the nude and immunodeficient phenotypes. NuRabbits support iPSC teratoma assay and ...
Genome engineering technologies in rabbits
(Journal of Biomedical Research, 2020-01-01)
The rabbit has been recognized as a valuable model in various biomedical and biological research fields because of its intermediate size and phylogenetic proximity to primates. However, the technology for precise genome ...
Improving the genome assembly of rabbits with long-read sequencing
(Elsevier, 2021-09-01)
The European rabbit (Oryctolagus cuniculus) is important as a biomedical model given its unique features in immunity and metabolism. The current reference genome OryCun2.0 established with whole-genome shotgun sequencing ...
Gene Editing in Rabbits: Unique Opportunities for Translational Biomedical Research
(Frontiers, 2021-01-28)
The rabbit is a classic animal model for biomedical research, but the production of gene targeted transgenic rabbits had been extremely challenging until the recent advent of gene editing tools. More than fifty gene knockout ...
Intestinal dysbiosis in young cystic fibrosis rabbits
(MDPI, 2021-03-01)
Individuals with cystic fibrosis (CF) often experience gastrointestinal (GI) abnormalities. In recent years, the intestinal microbiome has been postulated as a contributor to the development of CF-associated GI complications, ...
Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering
(MDPI, 2022-07-01)
CRISPR–Cas9 is the state-of-the-art programmable genome-editing tool widely used in many areas. For safe therapeutic applications in clinical medicine, its off-target effect must be dramatically minimized. In recent years, ...
Production of CFTR-ΔF508 Rabbits.
(Frontiers, 2021-01-01)
Cystic Fibrosis (CF) is a lethal autosomal recessive disease caused by mutations in the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR). The most common mutation is the deletion of phenylalanine ...
Effects of survival motor neuron protein on germ cell development in mouse and human
(MDPI, 2021-01-02)
Survival motor neuron (SMN) is ubiquitously expressed in many cell types and its encoding gene, survival motor neuron 1 gene (SMN1), is highly conserved in various species. SMN is involved in the assembly of RNA spliceosomes, ...
Diversity and complexity of the large surface protein family in the compacted genomes of multiple pneumocystis species
(American Society for Microbiology, 2020-03-01)
Pneumocystis, a major opportunistic pathogen in patients with a broad range of immunodeficiencies, contains abundant surface proteins encoded by a multicopy gene family, termed the major surface glycoprotein (Msg) gene ...
Phenotypes of CF rabbits generated by CRISPR/Cas9-mediated disruption of the CFTR gene
(American Society for Clinical Investigation, 2021-01-11)
Existing animal models of cystic fibrosis (CF) have provided key insights into CF pathogenesis but have been limited by short lifespans, absence of key phenotypes, and/or high maintenance costs. Here, we report the ...