Now showing items 41-50 of 173
Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning
(Elsevier, 1983-11)
A novel plasmid vector that is able to replicate both in Escherichia coli and in Streptococcus sanguis is described. This 9.2-kb plasmid, designated pVA856, carries Cmr, Tcr and Emr determinants that are expressed in E. ...
Comparative analysis of prototype two-component systems with either bifunctional or monofunctional sensors: differences in molecular structure and physiological function
(Blackwell Science Ltd, 2003-04)
Signal transduction by a traditional two-component system involves a sensor protein that recognizes a physiological signal, autophosphorylates and transfers its phosphate, and a response regulator protein that receives the ...
Isolation and structure of the Streptococcus faecalis sex pheromone, cAM373
(Elsevier, 1986-09-29)
The Streptococcus faecalis sex pheromone, cAM373, which induces a mating response of donor cells harboring plasmid pAM373 and is also produced by Staphylococcus aureus, was isolated and its structure determined. Supernatant ...
A cloning vector able to replicate in Escherichia coli and Streptococcus sanguis
(Elsevier, 1982-10)
A plasmid that is able to replicate in both Escherichia coli and Streptococcus sanguis has been constructed by the in vitro joining of the pACYC184 (Cmr Tcr) and pVA749 (Emr) replicons. This plasmid, designated pVA838, is ...
Am E. coli gene product required for [lambda] site-specific recombination
(Elsevier, 1980-07)
We report characteristics of himA mutations of E. coli, selected for their inability to support the site-specific recombination reaction involved in the formation of lysogens by bacteriophage [lambda]. The himA allele lies ...
Identification and characterization of the cAMP binding proteins of yeast by photoaffinity labeling
(Elsevier, 1979-10-12)
Contrary to previous reports, we find the molecular weight of the major cAMP-binding protein in to be 54,000 daltons. A number of lower molecular weight proteins have also been identified and are believed to be breakdown ...
Cooperative effects of bacterial mutations affecting [lambda] N gene expression : II. Isolation and characterization of mutations in the rif region
(Elsevier, 1976-08)
We report the isolation and initial characterization of a class of mutations, Snu, that map near the rif locus on the Escherichia coli chromosome. Snu mutations inhibit the growth of phage [lambda], an effect primarily ...
Characterization of the Determinant (traB) Encoding Sex Pheromone Shutdown by the Hemolysin/Bacteriocin Plasmid pAD1 in Enterococcus faecalis
(Elsevier, 1994-03)
pAD1 is a hemolysin/bacteriocin plasmid originally identified in Enterococcus faecalis DS16. It encodes a mating response to a peptide sex pheromone, cAD1, secreted by recipient bacteria. Once pAD1 is acquired, production ...
Bacteriophage Lambda N-Dependent Transcription Antitermination : Competition for an RNA Site May Regulate Antitermination
(Elsevier, 1994-02-10)
Bacteriophage [lambda] controls the expression of its early genes in a temporal manner by a series of transcription termination and antitermination events. This antitermination requires the [lambda] N protein as well as ...
Temperature dependent alteration in bacterial protein composition
(Elsevier, 1975-02-03)
When different strains of or are grown at 30[deg] or at 42[deg] and their proteins are compared using SDS-polyacrylamide gel electrophoresis, differences are observed in the protein composition of the cells. In particular, ...