Now showing items 1-10 of 17
Two routes for synthesis of phosphoenolpyruvate from C4-dicarboxylic acids in
(Elsevier, 1974-08-19)
Mutants of defective in both phosphoenolpyruvate carboxykinase and phosphoenolpyruvate synthetase are unable to use C4-dicarboxylic acids such as succinate and malate as carbon and energy sources for growth. Revertants ...
Effects of rifampicin, streptolydigin and actinomycin D on the replication of Col E1 plasmid DNA in Escherichia coli
(Elsevier, 1973-04-15)
We recently reported (Clewell et al., 1972) on an inhibitory effect of rifampicin on Col E1 plasmid replication. The present study represents a further characterization of this phenomenon as well as a study of the effects ...
Identification and characterization of the cAMP binding proteins of yeast by photoaffinity labeling
(Elsevier, 1979-10-12)
Contrary to previous reports, we find the molecular weight of the major cAMP-binding protein in to be 54,000 daltons. A number of lower molecular weight proteins have also been identified and are believed to be breakdown ...
Temperature dependent alteration in bacterial protein composition
(Elsevier, 1975-02-03)
When different strains of or are grown at 30[deg] or at 42[deg] and their proteins are compared using SDS-polyacrylamide gel electrophoresis, differences are observed in the protein composition of the cells. In particular, ...
Patterns of protein synthesis in E. coli: a catalog of the amount of 140 individual proteins at different growth rates
(Elsevier, 1978-05)
The amount of 140 individual proteins of E. coli B/r was measured during balanced growth in five different media. The abundance of each protein was determined from its absolute amount in 14C-glucose-minimal medium and a ...
Gene N regulator function of phage [lambda]imm21: Evidence that a site of N action differs from a site of N recognition
(Elsevier, 1973-12-25)
We report the isolation and characterization of a new mutation in the hybrid phage [lambda]imm21. Both genetic and physiological studies demonstrate that this new mutation, N21-1, is similar to N mutations of phage [lambda]. ...
Isolation of mutants of Escherichia coli lacking NAD- and NADP-linked malic enzyme activities
(Elsevier, 1975-07-22)
SummaryWhen Escherichia coli utilizes C4-dicarboxylic acids as sole sources of carbon and energy for growth, phosphoenolpyruvate is synthesized by means of phosphoenolpyruvate carboxykinase or by sequential action of malic ...
A criterion for using chloramphenicol to define different processes in the initiation of DNA synthesis in bacteria
(Elsevier, 1974-07)
A criterion is proposed which must be met in order to demonstrate that two different proteins are required for the initiation of DNA synthesis, with each protein being synthesized at a different time prior to initiation, ...
Feedforward inhibition in biosynthetic pathways: inhibition of the aminoacyl-tRNA synthetase by intermediates of the pathway
(Elsevier, 1979-04-21)
The stability of an amino acid biosynthetic pathway controlled by end-product inhibition is significantly improved if, in addition, the corresponding aminoacyl-tRNA synthetase is inhibited by an intermediate in the pathway. ...
Metabolic regulation of aminoacyl-tRNA synthetase formation in bacteria
(Elsevier, 1972-10-17)
The cellular levels of several aminoacyl-tRNA synthetases vary with the rate of growth of , and of , in different media. Over a seven-fold range in growth rate the levels of arginyl- and of valyl-tRNA synthetase change ...