Now showing items 1-10 of 26
Transtubular bioreactor: A perfusion device for mammalian cell cultivation
(Kluwer Academic Publishers; Science & Technology Letters ; Springer Science+Business Media, 1989-01)
A transtubular mammalian cell perfusion chamber has been developed that allows separate delivery and control of medium and gas flow to the cells. The hydrodynamic and mass transfer characteristics of the reactor are studied ...
Stimulation of fibroblast proliferation by thrombospondin
(Elsevier, 1989-08-30)
Thrombospondin purified from human platelets was examined for its ability to promote proliferation of human dermal fibroblasts. The results show that thrombospondin could stimulate the incorporation of [3H]thymidine by ...
Differing calcium requirements for regulatory effects of ATP, ATP[gamma]S and adenosine on O2 responses of human neutrophils
(Elsevier, 1988-07-29)
In formyl peptide stimulated human neutrophils the enhancement of O2 responses by ATP and ATP[gamma]S requires extracellular calcium. In contrast, the inhibitory effects of adenosine are independent of a calcium requirement. ...
The beta form is the dominant interleukin released by murine peritoneal macrophages
(Elsevier, 1989-04-14)
Using highly specific polyclonal antisera raised against recombinant murine IL-1 [alpha] and [beta], we performed solid-phase immunoabsorption studies on supernates of resident and adjuvant-elicited CBA/J mouse peritoneal ...
Production and utilization of extracellular matrix components by human melanocytes
(Elsevier, 1989-02)
Normal human melanocytes were separated from keratinocytes and maintained in culture using KGM medium supplemented with 12-O-tetradecanoylphorbol acetate and cholera toxin. The melanocytes were examined for the production ...
Prostaglandin E1 and prostaglandin I2 modulation of superoxide production by human neutrophils
(Elsevier, 1983-06-15)
15(S)-15-methyl-prostaglandin E1 and prostaglandin I2 rapidly and reversibly inhibit formyl-methionyl-leucyl-phenylalanine induced superoxide production by human neutrophils. In contrast, 15(S)-15-methyl-prostaglandin E1 ...
Products of cultured neuroglial cells: II. The production of fibronectin by C6 glioma cells
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 1985-05)
The possibility of fibronectin production by C6 glioma cells was examined with assays which require protein synthesis. Proteins produced by C6 cells using radiolabeled amino acid precursors were tested for affinity to ...
Enzyme-linked lectin assay (ELLA) : II. Detection of carbohydrate groups on the surface of unfixed cells
(Elsevier, 1984-03)
An enzyme-linked lectin assay (ELLA) has been developed to detect specific carbohydrate units on the surface of unfixed cells. The assay may be read in standard ELISA plate readers, since the cell-bound enzyme-lectin ...
Arachidonic acid metabolites regulate interleukin-1 production
(Elsevier, 1985-04-30)
We have investigated the role of arachidonic acid metabolites in the regulation of interleukin-1 production by murine peritoneal macrophages. Indomethacin a potent inhibitor of prostaglandin synthesis caused a dose-dependent ...
Loss of hippocampal [3H]TCP binding in Alzheimer's disease
(Elsevier, 1987-03-09)
We have previously demonstrated a marked loss in (NMDA) receptors in the hippocampus and cerebral cortex of patients dying with dementia of the Alzheimer type (DAT). In addition, we have found that the dissociative ...