Now showing items 1-10 of 16
Studies on morpholinosphingolipids: Potent inhibitors of glucosylceramide synthase
(Elsevier, 1994-04-25)
Synthetic 1-morpholino-1-deoxyceramides were designed to inhibit glucosylceramide synthase. The most potent inhibitor 2a possesses the unnatural R,R-configuration of D-threo-sphingosine.
Composition of the aqueous phase of chromaffin granules
(Elsevier, 1979-09-20)
Nuclear magnetic resonance spectroscopy has been used to determine the composition of the aqueous phase of bovine chromaffin granules. Relative concentrations of catecholamines (epinephrine plus norepinephrine), ATP and ...
Isolation and purification of a squamous cell carcinoma of the head and neck-associated antigen identified by autologous antibody
(Elsevier, 1993-04-30)
We have previously shown that detection of autologous antibody activity to squamous cell carninoma of the head and neck many be augmented by dissociation in immune complexes. Western blot analysis with autologous antibody ...
The effect of hemicholinium-3 on the in vivo formation of cerebral phosphatidylcholine
(Elsevier, 1970-02-10)
The effect of hemicholinium-3 on the incorporation of [Me-18C]choline into the phospholipids of the subcellular fractions of the canine caudate nucleus was studied. A correlation between the amount of hemicholinium-3 ...
Unimpaired formation of hormone-stimulated inositol triphosphate in human mesangial cells under hyperglycemic conditions
(Elsevier, 1992-03-20)
The relationship between bulk cellular myo-inositol content and phosphatidylinositol metabolism was evaluated in a human mesangial cell line under euglycemic and hyperglycemic conditions. Mesangial cells maintained in high ...
Further development and validation of empirical scoring functions for structure-based binding affinity prediction
(Kluwer Academic Publishers; Springer Science+Business Media, 2002-01)
New empirical scoring functions have been developed to estimate the binding affinity of a given protein-ligand complex with known three-dimensional structure. These scoring functions include terms accounting for van der ...
A CE assay for the detection of agonist-stimulated adenylyl cyclase activity
(WILEY-VCH Verlag, 2007-06)
A CE assay was developed for the detection of adenylyl cyclase (AC) activity stimulated at the AC and G protein-coupled receptor (GPCR) level. In the assay, cell membranes overexpressing GPCR and/or AC were incubated with ...
Partial purification and properties of a chromatin-associated phosphoprotein kinase from rat liver nuclei
(Elsevier, 1976-01-23)
A phosphoprotein kinase (EC 2.7.1.37) KIVb, from rat liver nuclei, was purified 75-fold by phosphocellulose chromatography and gel filtration on Sephadex G-200. The enzyme, which has an apparent molecular weight of 55 000, ...
The phosphatidylinositol synthase of proximal tubule cells
(Elsevier, 1990-05-01)
Phosphatidylinositol (PI) is a precursor for an important class of phospholipids, the phosphatidylinositol polyphosphates. Because renal myo-inositol levels may vary under both physiological (e.g., antidiuretic) and ...
Measurement of dissociation rate of biomolecular complexes using CE
(WILEY-VCH Verlag, 2009-02)
Fluorescence anisotropy (FA), non-equilibrium CE of equilibrium mixtures (NECEEM) and high-speed CE were evaluated for measuring dissociation kinetics of peptide–protein binding systems. Fyn-SH3-SH2, a protein construct ...