Now showing items 51-60 of 100
Rapid identification and screening of proteins from whole cell lysates of human erythroleukemia cells in the liquid phase, using non-porous reversed phase high-performance liquid chromatography separations of proteins followed by multi-assisted laser desorption/ionization mass spectrometry analysis and sequence database searching
(John Wiley & Sons, Ltd., 1998-12-30)
Non-porous reversed phase (NPRP) high-performance liquid chromatography (HPLC) has been used as a rapid method to separate proteins from whole cell lysates of human erythroleukemia (HEL) cells. Using phosphate-buffered ...
Stability of the contractile assembly and Ca 2+ - activated tension in adenovirus infected adult cardiac myocytes
(Kluwer Academic Publishers; Springer Science+Business Media, 1998-04)
Adenovirus-mediated gene transfer into adult cardiac myocytes in primary culture is a potentially useful method to study the structure and function of the contractile apparatus. However, the consequences of adenovirus ...
Adoptive Immunotherapy of Cancer with Activated Lymph Node Cells Primed In Vivo with Autologous Tumor Cells Transduced with the GM-CSF Gene University of Michigan, Ann Arbor, Michigan
(Mary Ann Liebert, Inc., publishers, 1996-04-10)
NON-TECHNICAL ABSTRACT This study involves the use of novel methods to treat patients with advanced cancer by manipulation of their immune system. Based on extensive animal studies, we know that the immune system has the ...
Biochemical and histopathological correlation in liver transplant: The first 180 days
(W.B. SaundersWiley Periodiocals, Inc., 1992-09)
It is not known whether the histopathology of the liver allograft can be predicted from biochemical measurements in serum with the same confidence as in the native liver. To answer this question we compared the histopathological ...
Systemic Delivery of the Interleukin-1 Receptor Antagonist Protein Using a New Strategy of Direct Adenoviral-Mediated Gene Transfer to Skeletal Muscle Capillary Endothelium in the Isolated Rat Hindlimb
(Mary Ann Liebert, Inc., publishers, 1996-10-01)
Overview summary Here we report highly efficient adenoviral-mediated gene transfer to the rat skeletal muscle capillary endothelium and muscle fibers during vascular isolation of the hindlimb. Gene transfer over a 109–1012 ...
Evaluation of the right atrial venous oxygen saturation as a physiologic monitor in a neonatal model
(Elsevier, 1993-07)
Pulmonary artery (PA) mixed venous saturation (SO2) has become a crucial monitor in the adult intensive care unit, but is not used in neonates because of the difficulty in PA catheterization. We evaluated the possibility ...
Effect of controlled hemorrhage on tissue and serum cefazolin clearance
(Elsevier, 1991-09)
Effects of blood loss on tissue and serum antibiotic levels were investigated in 30 New Zealand white rabbits. Studies were conducted over a 3.5-hr period after intravenous administration of cefazolin, 30 mg/kg, in control ...
Animal Models for the Study of Lower Extremity Chronic Venous Disease: Lessons Learned and Future Needs
(Springer-Verlag; by Annals of Vascular Surgery Inc., 1998-09)
Genetic Vaccination-Induced Immune Responses to the Human Immunodeficiency Virus Protein Rev: Emergence of the Interleukin 2-Producing Helper T Lymphocyte
(Mary Ann Liebert, Inc., publishers, 1998-10-10)
Overview summary The immune system poses a major obstacle to the long-term success of in vivo gene therapies. Immune responses to foreign transgene products and/or the vectors that facilitate gene transfer may neutralize ...
Selective resonance enhanced multiphoton ionization of aromatic polymers in supersonic beam mass spectrometry
(Elsevier, 1991-07-15)
Various polymers have been studied using a pulsed laser evaporation method for volatilizing these species and entraining them into a supersonic jet expansion followed by resonance enhanced multiphoton ionization (REMPI) ...